Wang S L, Ribar T J, Means A R
Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA.
Cell Growth Differ. 2001 Jul;12(7):351-61.
Ca(2+)/calmodulin-dependent protein kinase IV (CaMKIV) is a monomeric, multifunctional serine/threonine protein kinase that is expressed in subanatomic regions of the central and peripheral nervous system, T lymphocytes, and male germ cells. It is frequently localized to the nucleus, where it serves as a mediator of Ca(2+)-dependent gene expression. Although CaMKIV expression in the adult rat central nervous system and thymus has been described, little is known about the embryonic expression of murine CaMKIV. Here we report a thorough embryonic expression study of CaMKIV mRNA from embryonic day 9.5 through postnatal day 1. Expression patterns during embryonic development are significantly different from those of adults, suggesting specific roles for CaMKIV during development. Regions of high CaMKIV mRNA expression include thymic and bone cartilage primordia as well as specific cranial nerve ganglia (trigeminal, vestibulocochlear, and glossopharyngeal), thalamus, and dorsal root ganglia. This pattern of expression is chronologically consistent with periods of extensive cellular differentiation, proliferation, or neuronal survival selection and shows a predilection for neural crest-derived cells. These trends, along with recent studies in the CaMKIV null mouse, suggest that CaMKIV may play an important physiological role in cellular differentiation during embryogenesis.
钙/钙调蛋白依赖性蛋白激酶IV（CaMKIV）是一种单体多功能丝氨酸/苏氨酸蛋白激酶，在中枢和外周神经系统的亚解剖区域、T淋巴细胞和雄性生殖细胞中表达。它常定位于细胞核，在其中作为钙依赖性基因表达的介质。虽然已经描述了成年大鼠中枢神经系统和胸腺中CaMKIV的表达，但对于小鼠CaMKIV的胚胎表达知之甚少。在此，我们报告了从胚胎第9.5天到出生后第1天对CaMKIV mRNA进行的全面胚胎表达研究。胚胎发育期间的表达模式与成年期明显不同，表明CaMKIV在发育过程中具有特定作用。CaMKIV mRNA高表达区域包括胸腺和骨软骨原基以及特定的颅神经节（三叉神经节、前庭蜗神经节和舌咽神经节）、丘脑和背根神经节。这种表达模式在时间上与广泛的细胞分化、增殖或神经元存活选择时期一致，并且显示出对神经嵴衍生细胞的偏好。这些趋势，连同最近对CaMKIV基因敲除小鼠的研究，表明CaMKIV可能在胚胎发生过程中的细胞分化中发挥重要的生理作用。
