Takase-Yoden Sayaka, Watanabe Rihito
Institute of Life Science, Soka University, Tangi-cho 1-236, Hachioji, Tokyo 192-8577, Japan1.
J Gen Virol. 2001 Aug;82(Pt 8):1815-1820. doi: 10.1099/0022-1317-82-8-1815.
Friend murine leukaemia virus (FrMLV) FrC6 clone A8 causes spongiform degeneration in the central nervous system (CNS) of newborn but not 3-week-old rats. To assess whether expression of the ecotropic MLV receptor (CAT-1) in the CNS correlates with the pathogenicity of the A8 virus, we generated an anti-CAT-1 antibody raised against a synthetic peptide that corresponds to the carboxyl-terminal amino acid sequence of CAT-1. In the CNS of newborn and 3 to 4-week-old rats, a strong immunoreactivity against the antibody was detected in most of the endothelial cells. However, almost no expression of CAT-1 was detected in the CNS of 21-week-old rats. In newborn rats, many parenchymal cells in the brain as well as the vascular wall expressed CAT-1 antigen. These findings suggest that retrovirus receptor-bearing glial cells contribute to the neuropathogenesis of MLV, including clone A8, which induces spongiosis in rats only when inoculated into newborns.
Friend小鼠白血病病毒（FrMLV）FrC6克隆A8可导致新生大鼠而非3周龄大鼠的中枢神经系统（CNS）发生海绵状变性。为了评估嗜亲性MLV受体（CAT-1）在中枢神经系统中的表达是否与A8病毒的致病性相关，我们制备了一种抗CAT-1抗体，该抗体是针对与CAT-1羧基末端氨基酸序列相对应的合成肽产生的。在新生大鼠以及3至4周龄大鼠的中枢神经系统中，大多数内皮细胞均检测到针对该抗体的强免疫反应性。然而，在21周龄大鼠的中枢神经系统中几乎未检测到CAT-1的表达。在新生大鼠中，脑实质细胞以及血管壁均表达CAT-1抗原。这些发现表明，携带逆转录病毒受体的神经胶质细胞参与了MLV的神经发病机制，包括克隆A8，该病毒只有接种到新生大鼠体内时才会诱导大鼠发生海绵样变。
