Martianov I, Fimia G M, Dierich A, Parvinen M, Sassone-Corsi P, Davidson I
Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/ULP, Illkirch, C.U. de Strasbourg, France.
Mol Cell. 2001 Mar;7(3):509-15. doi: 10.1016/s1097-2765(01)00198-8.
Metazoan genomes encode two related proteins, TBP and the TBP-like factor (TLF/TRF2), sharing a highly conserved saddle-like domain. TLF is highly expressed in a finely regulated pattern in the mouse testis during spermatogenesis. The murine TLF gene has been inactivated using homologous recombination. TLF-/- mice are viable, but mutant male mice are sterile due to a late, complete arrest of spermiogenesis. In mutant animals, spermatogonia and spermatocytes develop normally, but round spermatids undergo apoptosis at step 7. Although the expression of the transcriptional activator CREM and many other postmeiotic genes was unaltered in TLF null mice, several spermiogenesis genes transcribed in late round spermatids appeared to be under TLF control. Hence, TLF is not required for embryonic development in the mouse but is essential for spermiogenesis.
后生动物基因组编码两种相关蛋白，即TBP和TBP样因子（TLF/TRF2），它们共享一个高度保守的鞍状结构域。TLF在小鼠睾丸精子发生过程中以精细调控的模式高度表达。利用同源重组使小鼠TLF基因失活。TLF基因敲除小鼠是可存活的，但突变雄性小鼠由于精子发生后期的完全停滞而不育。在突变动物中，精原细胞和精母细胞发育正常，但圆形精子细胞在第7阶段发生凋亡。尽管转录激活因子CREM和许多其他减数分裂后基因的表达在TLF基因敲除小鼠中未改变，但一些在圆形精子细胞后期转录的精子发生基因似乎受TLF调控。因此，TLF对小鼠胚胎发育不是必需的，但对精子发生至关重要。
