小鼠精子发生过程中TBP相关因子2（TRF2）的细胞和阶段特异性高水平表达。

Cell- and stage-specific high-level expression of TBP-related factor 2 (TRF2) during mouse spermatogenesis.

作者信息

Zhang D, Penttila T L, Morris P L, Roeder R G

机构信息

Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, New York, NY 10021, USA.

出版信息

Mech Dev. 2001 Aug;106(1-2):203-5. doi: 10.1016/s0925-4773(01)00439-7.

DOI:10.1016/s0925-4773(01)00439-7

PMID:11472857

Abstract

Mice lacking the TBP-related factor 2 (TRF2) gene, which is highly expressed in the testis, have a severe defect in spermiogenesis. Here we show that the expression of TRF2 is both cell type- and stage-specific. TRF2 expression was first detected in the late pachytene spermatocytes at stage VIII and increased throughout the subsequent stages. After meiotic divisions, the TRF2 expression declined continuously in round spermatids during progression from stage I to stage V. This observation is consistent with an essential regulatory role of TRF2 in male germ cell differentiation during spermatogenesis.

摘要

缺乏在睾丸中高表达的TBP相关因子2（TRF2）基因的小鼠在精子发生过程中存在严重缺陷。在这里，我们表明TRF2的表达具有细胞类型和阶段特异性。TRF2的表达首先在VIII期的粗线期晚期精母细胞中检测到，并在随后的阶段中持续增加。减数分裂后，在圆形精子细胞从I期到V期的发育过程中，TRF2的表达持续下降。这一观察结果与TRF2在精子发生过程中对雄性生殖细胞分化的重要调节作用一致。