Yu J, Zhang L, Hwang P M, Kinzler K W, Vogelstein B
The Johns Hopkins Oncology Center, The Johns Hopkins Medical Institutions, Baltimore, Maryland 21231, USA.
Mol Cell. 2001 Mar;7(3):673-82. doi: 10.1016/s1097-2765(01)00213-1.
Through global profiling of genes that were expressed soon after p53 expression, we identified a novel gene termed PUMA (p53 upregulated modulator of apoptosis). The protein encoded by PUMA was found to be exclusively mitochondrial and to bind to Bcl-2 and Bcl-X(L) through a BH3 domain. Exogenous expression of PUMA resulted in an extremely rapid and profound apoptosis that occurred much earlier than that resulting from exogenous expression of p53. Based on its unique expression patterns, p53 dependence, and biochemical properties, PUMA may be a direct mediator of p53-associated apoptosis.
通过对p53表达后不久即表达的基因进行全基因组分析，我们鉴定出一个名为PUMA（p53上调的凋亡调节因子）的新基因。发现由PUMA编码的蛋白质仅存在于线粒体中，并通过一个BH3结构域与Bcl-2和Bcl-X(L)结合。PUMA的外源性表达导致极其快速且深刻的凋亡，其发生时间比p53外源性表达所导致的凋亡要早得多。基于其独特的表达模式、对p53的依赖性及生化特性，PUMA可能是p53相关凋亡的直接介导因子。
