Dong Yihan, Qiao Qiufang, Guo Shaomin, Chen Ruibing, Lin Tianyu, Liu Xinyu, Li Jiaxin, Liu Shiming, Jiang Huamao, Wang Yong, Yue Dan, Wang Rui
School of Medical Technology, Tianjin Medical University, Tianjin 300203, China.
Department of Blood Transfusion, Cangzhou Hospital of Integrated Traditional and Western Medicine, Cangzhou 061012, China.
Int J Med Sci. 2025 May 30;22(11):2721-2737. doi: 10.7150/ijms.107055. eCollection 2025.
In this study, we analyzed the expression levels of ATP-binding cassette transporter G1 (ABCG1) in clear cell renal cell carcinoma (ccRCC) to assess its significance for early diagnosis and its role in the disease's biological processes. ABCG1 expression levels in ccRCC were determined by analyzing The Cancer Genome Atlas (TCGA) database, as well as immunohistochemical staining and western blot. The correlation between the ABCG1 expression level and the clinicopathological stage and diagnostic prognosis of ccRCC were analyzed by the TCGA database. The ABCG1 stable knockout cell line and functional experiments using ABCG1 inhibitors were constructed to verify the effect of reduced ABCG1 expression on the ccRCC cell function. In addition, the pathways and genes affected by ABCG1 were analyzed by RNA-seq. Analysis of the TCGA database demonstrated, as well as immunohistochemical staining and western blot detection, confirmed that ABCG1 was significantly elevated in ccRCC. The expression level of ABCG1 was correlated with the clinicopathological stage of ccRCC. The Cox regression analysis showed that ABCG1 could be used as an independent prognostic marker for ccRCC. And decreasing ABCG1 expression decreased the proliferation, migration, and invasion abilities of ccRCC cells, with increased apoptosis. In addition, analysis of ABCG1-related differentially expressed genes (DEGs) showed that ABCG1 positively regulates specific proliferation-related genes and negatively affects those associated with apoptosis. In summary, these findings highlight the critical role of ABCG1 in ccRCC progression and suggest its potential as a biomarker for diagnosis and prognosis.
在本研究中，我们分析了三磷酸腺苷结合盒转运体G1（ABCG1）在透明细胞肾细胞癌（ccRCC）中的表达水平，以评估其对早期诊断的意义及其在该疾病生物学过程中的作用。通过分析癌症基因组图谱（TCGA）数据库以及免疫组织化学染色和蛋白质印迹法来确定ccRCC中ABCG1的表达水平。利用TCGA数据库分析ABCG1表达水平与ccRCC临床病理分期及诊断预后之间的相关性。构建ABCG1稳定敲除细胞系并使用ABCG1抑制剂进行功能实验，以验证ABCG1表达降低对ccRCC细胞功能的影响。此外，通过RNA测序分析受ABCG1影响的信号通路和基因。TCGA数据库分析以及免疫组织化学染色和蛋白质印迹检测均证实，ABCG1在ccRCC中显著升高。ABCG1的表达水平与ccRCC的临床病理分期相关。Cox回归分析表明，ABCG1可作为ccRCC的独立预后标志物。降低ABCG1表达可降低ccRCC细胞的增殖、迁移和侵袭能力，并增加细胞凋亡。此外，对ABCG1相关差异表达基因（DEG）的分析表明，ABCG1正向调节特定的增殖相关基因，并对与凋亡相关的基因产生负面影响。总之，这些发现突出了ABCG1在ccRCC进展中的关键作用，并表明其作为诊断和预后生物标志物的潜力。
