卵胞浆内单精子注射后获得的人类胚胎采用慢速冷却或超快速冷却程序进行冷冻保存的比较。

Comparison of the cryopreservation of human embryos obtained after intracytoplasmic sperm injection with a slow cooling or an ultrarapid cooling procedure.

作者信息

Mauri A L, Petersen C G, Baruffi R L, Ferreira R C, Franco J G

机构信息

Centre for Human Reproduction, Sinhá Junqueira Maternity Foundation, Rua D. Alberto Gonçalves 1500, 14085-100, Ribeirão Preto, SP, Brazil.

出版信息

J Assist Reprod Genet. 2001 May;18(5):257-61. doi: 10.1023/a:1016630721045.

DOI:10.1023/a:1016630721045

PMID:11464576

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3455333/

Abstract

PURPOSE

Our purpose was to compare an ultrarapid method (URM) modified with dimethyl sulfoxide (Me2SO) to a slow method (SM) with propanediol (PROH) for the cryopreservation of extra human embryos in a program of intracytoplasmic sperm injection (ICSI).

METHODS

The extra embryos of 160 patients were cryopreserved in a prospective and randomized manner (drawing lots) by a modified URM (3 M Me2SO/0.25 M sucrose/thawing in three sucrose gradients) (Group I) or by a SM (1.5 M Propanediol/program 0-Cryologic CL863) (Group II). A total of 103 cycles has been thawed thus far. The number of thawed cycles was 58 for group I and 45 for group II.

RESULTS

The mean age (group I, 31.3 +/- 4.5; group II, 31.9 +/- 4.3) did not differ between the groups (P = 0.38). The number of frozen embryos (group I, 6.6 +/- 3.2; group II, 6.5 +/- 3.2) was similar (P = 0.49) for the two groups, as was the number of thawed embryos (P = 0.52) (group I, 6.5 +/- 2.9; group II, 6.2 +/- 3). The survival rate was higher (P < 0.01) for group II (83.3 +/- 23%) than for group I (69.2 +/- 28.7%). The cleavage rate was also higher (P < 0.01) for group II (56.8 +/- 31%) compared with group I (24.2 +/- 22.4%). The number of embryos transferred did not differ (P = 0.14) between the groups (group I, 3.16 +/- 1.2; group II, 3.5 +/- 1.0). The implantation rate (group I, 6.3%; group II, 13.8%) was significantly different between groups (P = 0.034). Pregnancy rates per thawed and transferred cycle were higher for group II (33.3 and 36.6%, respectively) compared with group I (13.8 and 16%, respectively), and these differences were significant (P = 0.03 and P = 0.03, respectively).

CONCLUSION

The data obtained suggest that the SM is superior to the URM for the cryopreservation of extra embryos after ICSI.

摘要

目的

我们的目的是比较一种用二甲基亚砜（Me2SO）改良的超快速方法（URM）与一种使用丙二醇（PROH）的慢速方法（SM），用于在卵胞浆内单精子注射（ICSI）程序中冷冻保存多余的人类胚胎。

方法

160例患者的多余胚胎以前瞻性和随机方式（抽签）通过改良的URM（3M Me2SO/0.25M蔗糖/在三个蔗糖梯度中解冻）（第一组）或通过SM（1.5M丙二醇/程序0 - Cryologic CL863）（第二组）进行冷冻保存。到目前为止，共解冻了103个周期。第一组解冻周期数为58个，第二组为45个。

结果

两组之间的平均年龄（第一组，31.3±4.5；第二组，31.9±4.3）无差异（P = 0.38）。两组的冷冻胚胎数（第一组，6.6±3.2；第二组，6.5±3.2）相似（P = 0.49），解冻胚胎数也相似（P = 0.52）（第一组，6.5±2.9；第二组，6.2±3）。第二组的存活率（83.3±23%）高于第一组（69.2±28.7%）（P < 0.01）。第二组的卵裂率（56.8±31%）也高于第一组（24.2±22.4%）（P < 0.01）。两组之间移植的胚胎数无差异（P = 0.14）（第一组，3.16±1.2；第二组，3.5±1.0）。两组之间的着床率（第一组，6.3%；第二组，13.8%）有显著差异（P = 0.034）。第二组每个解冻和移植周期的妊娠率分别高于第一组（分别为33.3%和36.6%）和（分别为13.8%和16%），这些差异具有统计学意义（分别为P = 0.03和P = 0.03）。