Kennedy J F, Schreiber J R, Andreassen B K
Am J Obstet Gynecol. 1975 Aug 1;122(7):863-71. doi: 10.1016/0002-9378(75)90729-2.
A study was made of ovaries perfused in vitro with a liquid fluorocarbon that was stabilized in emulsion by a nonionic detergent to which tissue culture medium was added. A pulsatile flow was used in an organ preservation system under aseptic conditions. Glucose was utilized and lactate produced at a fairly constant rate for as long as 20 hours of perfusion. 14-C-progesterone and 14-C-17alphaOH-progesterone were synthesized from 14-C-acetate and estradiol was secreted. The production of these steroids by the perfused ovary appears to be responsive to gonadotropins.
对用液态氟碳体外灌注的卵巢进行了一项研究，该液态氟碳通过添加了组织培养基的非离子洗涤剂稳定在乳液中。在无菌条件下的器官保存系统中使用脉动流。在长达20小时的灌注过程中，葡萄糖被利用，乳酸以相当恒定的速率产生。从14-C-乙酸盐合成了14-C-孕酮和14-C-17α-羟基孕酮，并分泌了雌二醇。灌注卵巢产生这些类固醇似乎对促性腺激素有反应。
