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利用一个F2家系对影响鸡体重的QTL进行遗传定位。

Genetic mapping of the QTL affecting body weight in chickens using a F2 family.

作者信息

Tatsuda K, Fujinaka K

机构信息

Hyogo Prefectural Agricultural Institute, Kasai, Japan.

出版信息

Br Poult Sci. 2001 Jul;42(3):333-7. doi: 10.1080/00071660120055296.

DOI:10.1080/00071660120055296
PMID:11469552
Abstract
  1. To identify the quantitative trait loci (QTL) affecting growth in chickens, we carried out QTL analysis on chicken growth traits using a population of 227 F2 crosses between a Satsumadori (slow-growing, light-weight Japanese native breed used as a meat chicken) male and a White Plymouth Rock (early-maturing, heavy weight broiler). 2. We chose 78 microsatellite loci from 331 publicly available on 14 linkage groups, with respect to their utility and location. 3. Two QTLs affecting body weight at 13 and 16 weeks were mapped at 220 cM on chromosome 1 (LOD scores, 2.8 and 4.5, respectively, at 13 and 16 weeks), and at 60 cM on chromosome 2 (LOD scores, 6.2 and 8.1, respectively, at 13 and 16 weeks). 4. The closest loci to the QTLs were LEI71 on chromosome 1 and LMU13 and MCW184 on chromosome 2. 5. The sites of the QTLs agreed closely with those already reported. Therefore, it seems likely that QTLs affecting growth of chickens are located at these sites.
摘要
  1. 为了鉴定影响鸡生长的数量性状基因座(QTL),我们利用227个F2杂交群体(由一只萨摩鸡(一种生长缓慢、体重较轻的日本本土肉鸡品种)雄性与一只白普利茅斯洛克鸡(早熟、体重较重的肉鸡)杂交产生)对鸡的生长性状进行了QTL分析。2. 我们从14个连锁群上公开可用的331个微卫星位点中,根据其效用和位置选择了78个微卫星位点。3. 两个影响13周龄和16周龄体重的QTL分别定位在1号染色体上220厘摩处(13周龄和16周龄时的LOD分数分别为2.8和4.5),以及2号染色体上60厘摩处(13周龄和16周龄时的LOD分数分别为6.2和8.1)。4. 与这些QTL最接近的位点在1号染色体上是LEI71,在2号染色体上是LMU13和MCW184。5. 这些QTL的位点与已报道的位点高度一致。因此,影响鸡生长的QTL似乎位于这些位点。

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