八株产类胡萝卜素的杜氏盐藻（绿藻门）智利菌株的遗传多态性。

Genetic polymorphism in eight Chilean strains of the carotenogenic microalga Dunaliella salina Teodoresco (Chlorophyta).

作者信息

Gomez P I, Gonzalez M A

机构信息

Dpto. Biología Molecular, Facultad de Ciencias Biológicas, Universidad de Concepción, Casilla 160-C, Concepción, Chile.

出版信息

Biol Res. 2001;34(1):23-30. doi: 10.4067/s0716-97602001000100012.

DOI:10.4067/s0716-97602001000100012

PMID:11471520

Abstract

Eight Chilean strains of Dunaliella salina obtained within a restricted geographic range, but exhibiting a high variability in their morphology, rate of growth and carotenogenic capacity, were analyzed by Random Amplified Polymorphic DNA (RAPD-PCR) Twenty of the 50 random primers (D, P, OPA and OPD series) that were tested amplified reproducible bands and were useful for comparative analysis of the strains. Of 107 polymorphic genetic markers, 49 were strain-specific. A great genetic variability was found among the strains in spite of their geographic proximity. In addition, phenetic analysis of the data showed close agreement between the morphophysiological attributes and the genetic diversity of the strains.

摘要

对在有限地理范围内获得的8株智利盐生杜氏藻进行了分析，这些藻在形态、生长速率和产胡萝卜素能力方面表现出高度变异性。采用随机扩增多态性DNA（RAPD-PCR）技术，对50条随机引物（D、P、OPA和OPD系列）进行了测试，其中20条引物扩增出了可重复的条带，可用于菌株的比较分析。在107个多态性遗传标记中，49个是菌株特异性的。尽管这些菌株在地理上相近，但仍发现它们之间存在很大的遗传变异性。此外，对数据的聚类分析表明，菌株的形态生理特征与遗传多样性之间存在密切一致性。