Chen Yong, Ni Hanwen
Department of Agronomy, Quartermaster University of PLA, Changchun 130062, China.
Wei Sheng Wu Xue Bao. 2003 Aug;43(4):409-16.
Exserohilium monoseras is a potential agent for barnyardgrass (Echinochlao crus-galli) control. This study was to evaluate the genetic diversity of this fungus with 17 strains isolated from different rice growing regions of China by the random amplified polymorphic DNA (RAPD). Among 25 arbitrary primers, 20 primers could get enough amplified bands for all the strains. A total 239 products were amplified. Polymorphic bands were 95.8% of the total products. The 17 strains could be identified based on RAPD fingerprinting established in this study. Genetic distance calculation and cluster analysis resulted in great genetic variation among the strains. The genetic similarity was related with the morphology and the pathogenicity of the strains. The results showed that RAPD maker could be applied in the utilization and the identification of weed pathogen resources.
稻平脐蠕孢是稗草防治的潜在药剂。本研究采用随机扩增多态性DNA(RAPD)技术对从中国不同水稻种植区分离得到的17株该真菌进行遗传多样性评价。在25条随机引物中,20条引物能对所有菌株扩增出足够的条带。共扩增出239个产物。多态性条带占总产物的95.8%。基于本研究建立的RAPD指纹图谱可对17个菌株进行鉴定。遗传距离计算和聚类分析结果表明菌株间存在较大的遗传变异。遗传相似性与菌株的形态和致病性有关。结果表明,RAPD标记可应用于杂草病原菌资源的利用和鉴定。