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terrestre毛癣菌的磷酸甘油酯以及从阿波罗16号微生物生态评估装置中选出的一种表型。

Phosphoglycerides of Trichophyton terrestre and one phenotype selected from the Apollo 16 microbial ecology evaluation device.

作者信息

Sawyer R T, Deskins D C, Volz P A

出版信息

Appl Microbiol. 1975 May;29(5):658-62. doi: 10.1128/am.29.5.658-662.1975.

DOI:10.1128/am.29.5.658-662.1975
PMID:1147604
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC187053/
Abstract

Total lipid extracted from wild-type Trichophyton terrestre CDC-X285 was found to be 2.0 percent of the dry cell weight. The total lipid contained the following phospholipid components identified by silicic acid-impregnated thin-layer and paper chromatography: phosphatidyl inositol, phosphatidyl choline, phosphatidyl serine, and phosphatidic acid. The total lipid extracted from the phenotype T. terrestre 7048-1 isolated from the Apollo 16 Microbial Ecology Evaluation Device (MEED) was found to vary according to the time at which the phospholipids were extracted. The Trichophyton phenotype was selected from a cuvette housed in the MEED exposed to specific space parameters including ultraviolet light of known wavelengths and energy levels in deep space. The phospholipid components, identified in the phenotype were phosphatidyl ethanolamine and cardiolipin. The major lipid fraction was composed of digalactosyl diglyceride and monogalactosyl diglyceride. An unusual lipid was detected in the phenotype, which appeared to be sterol glycoside.

摘要

从野生型土生毛癣菌CDC-X285中提取的总脂质占干细胞重量的2.0%。通过硅酸浸渍薄层层析和纸层析鉴定,总脂质含有以下磷脂成分:磷脂酰肌醇、磷脂酰胆碱、磷脂酰丝氨酸和磷脂酸。从阿波罗16号微生物生态评估装置(MEED)分离出的土生毛癣菌7048-1表型中提取的总脂质,发现其会根据提取磷脂的时间而有所不同。该土生毛癣菌表型是从MEED中的一个比色皿中选取的,该比色皿暴露于特定的空间参数下,包括已知波长和深空能量水平的紫外线。在该表型中鉴定出的磷脂成分是磷脂酰乙醇胺和心磷脂。主要脂质部分由二半乳糖基甘油二酯和单半乳糖基甘油二酯组成。在该表型中检测到一种不寻常的脂质,似乎是甾醇糖苷。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a564/187053/0f98df06ed88/applmicro00023-0119-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a564/187053/cbb2a6bcbc72/applmicro00023-0118-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a564/187053/0f98df06ed88/applmicro00023-0119-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a564/187053/cbb2a6bcbc72/applmicro00023-0118-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a564/187053/0f98df06ed88/applmicro00023-0119-a.jpg

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本文引用的文献

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A rapid method of total lipid extraction and purification.一种快速的总脂质提取与纯化方法。
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Occurrence of squalene, di- and tetrahydrosqualenes, and vitamin MK8 in an extremely halophilic bacterium, Halobacterium cutirubrun.在极端嗜盐细菌——深红嗜盐杆菌中鲨烯、二氢鲨烯和四氢鲨烯以及维生素MK8的存在情况。
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The antifungal activity of normal and host-compromised saliva on spaceflight fungal phenotypes.正常唾液和宿主受损唾液对航天飞行真菌表型的抗真菌活性。
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