Schroeder H W, Stipanovic R D
Appl Microbiol. 1975 May;29(5):706-7. doi: 10.1128/am.29.5.706-707.1975.
Aspergillus caepitosus Raper and Thom cultured on autoclaved cracked, yellow field corn produced 3-methyl-6-methoxy-8-hydroxy-3,4-dihydroisocoumarin which was extracted and then purified by silica gel columns. The physical and spectral properties of the colorless crystals from methylene chloride:hexane were consistent with those published for 3-methyl-6-methoxy-8-hydroxy-3,4-dihydroisocoumarin. Yield of high purity 3-methyl-6-methoxy-8-hydroxy-3,4-dihydroisocoumarin was about 140 mg of corn per kg.
在经高压灭菌的破碎黄色大田玉米上培养的头状曲霉Raper和Thom产生了3-甲基-6-甲氧基-8-羟基-3,4-二氢异香豆素，将其提取后通过硅胶柱进行纯化。从二氯甲烷：己烷中得到的无色晶体的物理和光谱性质与已发表的3-甲基-6-甲氧基-8-羟基-3,4-二氢异香豆素的性质一致。每千克玉米中高纯度3-甲基-6-甲氧基-8-羟基-3,4-二氢异香豆素的产量约为140毫克。
