Sargent L M, Nelson M A, Lowry D T, Senft J R, Jefferson A M, Ariza M E, Reynolds S H
Toxicology and Molecular Biology Branch, Health Effects Laboratory Division, National Institute of Occupational Safety and Health, Morgantown, West Virginia, USA.
Genes Chromosomes Cancer. 2001 Sep;32(1):18-25. doi: 10.1002/gcc.1162.
Chromosomal aberrations in malignant melanoma cells have been reported using standard chromosome banding analysis and comparative genomic hybridization. To identify marker chromosomes and translocations that are difficult to characterize by standard banding analysis, 15 early passage malignant melanoma cell lines were examined using spectral karyotyping. All 15 tumor cell lines had lost all or part of 1p and 10q. Losses of material on chromosome arms 4p (12/15), 6q (12/15), 9p (15/15), 12p (13/15), 12q (13/15), 13q (11/15), and 19q (14/15) were the next most frequent events. Gain of chromosome arms 1q (11/15), 6p (13/15), and 20q11 (14/15) was also observed. Interestingly, we identified translocations der(12)t(12;20)(q15;q11), der(19)t(10;19)(q23;q13), and der(12)t(12;19)(q13;q13) in 4/15 tumors. Three recurring translocations involving four of the most frequent break points were detected. The identification of recurring translocations and unique chromosome break points in melanoma will aid in the identification of the genes that are important in the neoplastic process.
已通过标准染色体显带分析和比较基因组杂交技术报道了恶性黑色素瘤细胞中的染色体畸变情况。为了识别那些难以通过标准显带分析进行特征描述的标记染色体和易位，使用光谱核型分析技术对15个早期传代的恶性黑色素瘤细胞系进行了检测。所有15个肿瘤细胞系均丢失了1p和10q的全部或部分区域。染色体臂4p（12/15）、6q（12/15）、9p（15/15）、12p（13/15）、12q（13/15）、13q（11/15）和19q（14/15）上的物质丢失是接下来最常见的事件。还观察到染色体臂1q（11/15）、6p（13/15）和20q11（14/15）的获得。有趣的是，我们在4/15的肿瘤中识别出了der(12)t(12;20)(q15;q11)、der(19)t(10;19)(q23;q13)和der(12)t(12;19)(q13;q13)易位。检测到了涉及四个最常见断点中的三个反复出现的易位。黑色素瘤中反复出现的易位和独特的染色体断点的识别将有助于识别在肿瘤形成过程中重要的基因。
