Huang H, Xu J, Zhong N
Guangzhou Institute of Respiratory Disease, Gunangzhou 510120.
Zhonghua Jie He He Hu Xi Za Zhi. 1998 Oct;21(10):612-4.
To evaluate the significance of EG1 or EG2 positive eosinophils in relating to the severity of asthma.
EDTA-anticoagulated venous blood were treated by FOG method, and FTTC-conjugated monoclonal antibodies were added. Flow cytometry technique was used.
EG1 and EG2 positive eosinophils increased significantly in patients with acute asthma (n = 29) as compared with control (n = 13) and COPD (n = 12), P < 0.05. There were significant correlation of the mean fluorescence intensity(MFI) between EG1, EG2 and the degree of the symptom.
There is a significant increase of activated eosinophils in peripheral blood in asthmatics, the expression of EG1 and EG2 positive eosinophils correlated well with the severity of symptom in asthmatics. EG1 and EG2, as the marker for eosinophils activity, may be sensitive and specific in evaluating the severity of asthmatics symptom.
评估EG1或EG2阳性嗜酸性粒细胞与哮喘严重程度的相关性。
采用FOG法处理乙二胺四乙酸（EDTA）抗凝的静脉血，并加入异硫氰酸荧光素（FTTC）偶联的单克隆抗体。使用流式细胞术。
与对照组（n = 13）和慢性阻塞性肺疾病（COPD）组（n = 12）相比，急性哮喘患者（n = 29）中EG1和EG2阳性嗜酸性粒细胞显著增加，P < 0.05。EG1、EG2的平均荧光强度（MFI）与症状程度之间存在显著相关性。
哮喘患者外周血中活化嗜酸性粒细胞显著增加，EG1和EG2阳性嗜酸性粒细胞的表达与哮喘患者症状严重程度密切相关。EG1和EG2作为嗜酸性粒细胞活性标志物，在评估哮喘患者症状严重程度方面可能具有敏感性和特异性。
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