Moqbel R, Barkans J, Bradley B L, Durham S R, Kay A B
Department of Allergy and Clinical Immunology, National Heart and Lung Institute, London, U.K.
Clin Exp Allergy. 1992 Feb;22(2):265-73. doi: 10.1111/j.1365-2222.1992.tb03082.x.
A monoclonal antibody prepared against the eosinophil major basis protein (MBP) was compared with the anti-eosinophil cationic protein (ECP) antibodies (EG1 and EG2) in immunostaining of bronchial biopsies from atopic asthma and controls. Anti-MBP (designated BMK-13) did not cross-react with other eosinophil basic proteins (i.e. ECP, eosinophil peroxidase [EPO] or eosinophil-derived neurotoxin [EDN]) and stained more than 98% of peripheral blood eosinophils irrespective of their degree of activation. EG2 stained 15% of resting and 75% of activated eosinophils; EG1 recognized 74% and 78% of resting and activated cells, respectively. The numbers of BMK-13, EG1 or EG2-positive staining cells in bronchial biopsies from asthma were significantly greater than atopic non-asthmatics (P less than 0.02, P less than 0.01 and P less than 0.05, respectively) and normal non-atopic controls (P less than 0.001). For each of the various groups studied, the rank order for the number of eosinophils stained was BMK-13 greater than EG1 greater than EG2. BMK-13 stained significantly more cells from bronchial biopsies of atopic asthma and atopic non asthma when compared to EG2 (P less than 0.001 and P less than 0.05, respectively). Since only a proportion of BMK-13+ cells were EG2+, these results suggest that not all tissue eosinophils are actively secreting. Thus, BMK-13 can serve as a useful pan-eosinophil marker in tissue sections since it appears to stain most eosinophils.
将针对嗜酸性粒细胞主要碱性蛋白（MBP）制备的单克隆抗体与抗嗜酸性粒细胞阳离子蛋白（ECP）抗体（EG1和EG2）在特应性哮喘患者和对照组的支气管活检组织免疫染色中进行比较。抗MBP（命名为BMK-13）与其他嗜酸性粒细胞碱性蛋白（即ECP、嗜酸性粒细胞过氧化物酶[EPO]或嗜酸性粒细胞衍生神经毒素[EDN]）无交叉反应，且能使超过98%的外周血嗜酸性粒细胞染色，无论其活化程度如何。EG2能使15%的静息嗜酸性粒细胞和75%的活化嗜酸性粒细胞染色；EG1分别识别74%的静息细胞和78%的活化细胞。哮喘患者支气管活检组织中BMK-13、EG1或EG2阳性染色细胞的数量显著多于特应性非哮喘患者（分别为P<0.02、P<0.01和P<0.05）和正常非特应性对照组（P<0.001）。对于所研究的各个组，嗜酸性粒细胞染色数量的排序为BMK-13>EG1>EG2。与EG2相比，BMK-13对特应性哮喘和特应性非哮喘患者支气管活检组织中的细胞染色显著更多（分别为P<0.001和P<0.05）。由于只有一部分BMK-13+细胞为EG2+，这些结果表明并非所有组织嗜酸性粒细胞都在积极分泌。因此，BMK-13可作为组织切片中一种有用的全嗜酸性粒细胞标志物，因为它似乎能使大多数嗜酸性粒细胞染色。
