Malecki M, Przybyszewska M, Janik P
Department of Cell Biology, Cancer Center, Warsaw, Poland.
Arch Immunol Ther Exp (Warsz). 2001;49(3):243-6.
Vascular endothelial growth factor (VEGF) is an angiogenic cytokine with potential therapeutic applications in human diseases. It is a mitogen primarily for endothelial cells. The transfer of the cDNA encoding VEGF to ischemic tissues, which cannot be revascularized otherwise, represents a novel and promising approach to the treatment of vascular disorders. In this work the VEGF165 cDNA was cloned into the expression vector pSecTag2B. The activity of the construct was studied in cell culture as well as in vivo. Western blotting study showed that the cells transfected with the vector secreted significantly higher amounts of VEGF to the culture medium than the non-transfected cells. In vivo study revealed an increased number of new vessels in animals injected with vector encoding VEGF as compared with empty plasmid. Also, tumor cells transfected with the VEGF plasmid exhibited extensive vascularization.
血管内皮生长因子（VEGF）是一种血管生成细胞因子，在人类疾病中具有潜在的治疗应用。它主要是内皮细胞的促有丝分裂原。将编码VEGF的cDNA转移到否则无法实现血管再生的缺血组织中，代表了一种治疗血管疾病的新颖且有前景的方法。在这项工作中，VEGF165 cDNA被克隆到表达载体pSecTag2B中。在细胞培养以及体内研究了该构建体的活性。蛋白质印迹研究表明，与未转染的细胞相比，用该载体转染的细胞向培养基中分泌的VEGF量显著更高。体内研究显示，与空质粒相比，注射编码VEGF的载体的动物体内新血管数量增加。此外，用VEGF质粒转染的肿瘤细胞表现出广泛的血管化。
