Fu Y, Niu D, Ruan H, Luo J, Chen G, Yu X P, Zhang Y P
Animal Science College of Zhejiang University, Hangzhou 310029, China.
Yi Chuan Xue Bao. 2001;28(7):606-13.
A total of 539 bases of mitochondrial DNA D-loop region of five native chicken breeds of Zhejiang province and the white leghorn chicken (as the control) were sequenced by DNA sequencing technique, and the phylogenetic trees of the chicken breeds were constructed. The results showed that 24 variation sites i.e. 4.45% sequence divergence were detected among the 30 DNA sequences, and the six breeds belonged to two different maternal lineage, one included Xianju chicken and White Leghorn chicken which had the same maternal origin, the other included Lingkun chicken, Baiyiner chicken, Wugu chicken and Xiaoshan chicken which had the same matriarchic ancestor. Among the latter lineage, Lingkun chicken, Baiyiner chicken and Wugu chicken had a closer relationship to each other than to Xiaoshan chicken.
采用DNA测序技术对浙江省5个地方鸡种和白来航鸡(对照)线粒体DNA D-loop区的539个碱基进行测序,并构建了这些鸡种的系统发育树。结果显示,在30条DNA序列中检测到24个变异位点,即序列分歧率为4.45%,6个鸡种分属于两个不同的母系世系,一个世系包括仙居鸡和白来航鸡,它们具有相同的母系起源;另一个世系包括灵昆鸡、白耳鸡、乌骨鸡和萧山鸡,它们有相同的母系祖先。在后一个世系中,灵昆鸡、白耳鸡和乌骨鸡之间的关系比它们与萧山鸡的关系更为密切。