Nwachukwu S C, James P, Gurney T R
Department of Botany and Microbiology, University of Lagos, Nigeria.
J Environ Biol. 2001 Jan;22(1):29-36.
The impacts of crude oil on the germination, growth and morphology of cress seeds (Lepidium sp.) after bioremediation of agricultural soil polluted with crude petroleum using "adapted" Pseudomonas putida (PP) were examined for 15 days. At day 15 there was 100% germination in the untreated control samples, the mean height of the seedlings was 75.8 +/- 2.6 mm and all appeared to have grown morphologically normal. In the experimental samples treated with oil and PP inoculation, there was 98% germination and the seedlings reached a height of 63.8 +/- 6.9 mm; again, morphologically the seedlings appeared normal. However, in the control samples treated with oil but without PP inoculation, there was 31-38% germination and seedling heights of 34.2 +/- 11.4-42.3 +/- 8.5 mm with abnormal morphology. Treatment of oil-impacted agricultural soil with PP as a bioremediation agent does produce soil which is capable of growing larger and healthier plants than where bioremediation has not taken place.
研究了用“适应性”恶臭假单胞菌（PP）对受原油污染的农业土壤进行生物修复后，原油对水芹种子（独行菜属）发芽、生长和形态的影响，为期15天。在第15天，未处理的对照样品发芽率为100%，幼苗平均高度为75.8±2.6毫米，且所有幼苗形态上似乎生长正常。在用油和PP接种处理的实验样品中，发芽率为98%，幼苗高度达到63.8±6.9毫米；同样，幼苗形态上看起来正常。然而，在仅用油处理而未接种PP的对照样品中，发芽率为31%-38%，幼苗高度为34.2±11.4-42.3±8.5毫米，且形态异常。用PP作为生物修复剂处理受油污染的农业土壤，确实能产生比未进行生物修复的土壤更有利于生长更大、更健康植物的土壤。
