Maffini M V, Ortega H H, Stoker C, Giardina R H, Luque E H, Muñoz de Toro M M
Department of Human Physiology, Faculty of Biochemistry and Biological Sciences, Universidad Nacional del Litoral, Santa Fe, Argentina.
Pathol Res Pract. 2001;197(7):487-92. doi: 10.1078/0344-0338-00116.
We evaluated the nuclear morphology, ploidy, bcl-2 expression and in situ apoptosis in sections of fine-needle aspiration (FNA) biopsy specimens of thirty-one randomly selected Stage B prostate carcinomas. Sections of paraffin-embedded pelleted cells obtained from FNA biopsy specimens were studied. Nuclear grade was determined according to the WHO system. Nuclear morphometry and DNA ploidy were carried out using an automated image analyzer. We used immunostaining and the TUNEL method to evaluate bcl-2 expression and in situ apoptosis. The median nuclear area increased with increasing nuclear grade. Ploidy analysis showed that 54.8% of tumors were diploid, 3.2% tetraploid and 41.9% aneuploid. Bcl-2 overexpression was found in 10 of 31 tumors. There was a significant positive correlation between bcl-2 expression and nuclear area (r(s): 0.45 p < 0.01). Nine of ten bcl-2-positive tumors had a nuclear area larger than the median of the series, and 70% of bcl-2-positive tumors were of the aneuploid type. The apoptotic index had a negative correlation with nuclear area, and the lowest indexes were found in aneuploid tumors. Bcl-2 expression showed a highly significant association with both parameters of high aggressiveness: nuclear size and aneuploidy. The combined evaluation of nuclear morphology, ploidy and cell survival parameters might better identify patients with poor prognosis among early stage prostate carcinomas diagnosed by FNA biopsies.
我们评估了31例随机选取的B期前列腺癌细针穿刺活检标本切片中的核形态、倍性、bcl-2表达及原位凋亡情况。研究了从细针穿刺活检标本中获取的石蜡包埋细胞团块的切片。核分级根据世界卫生组织系统确定。使用自动图像分析仪进行核形态测量和DNA倍性分析。我们采用免疫染色和TUNEL法评估bcl-2表达及原位凋亡。中位核面积随核分级增加而增大。倍性分析显示，54.8%的肿瘤为二倍体，3.2%为四倍体，41.9%为非整倍体。31例肿瘤中有10例发现bcl-2过表达。bcl-2表达与核面积之间存在显著正相关（斯皮尔曼相关系数：0.45，p<0.01）。10例bcl-2阳性肿瘤中有9例核面积大于该系列的中位数，70%的bcl-2阳性肿瘤为非整倍体类型。凋亡指数与核面积呈负相关，非整倍体肿瘤中的凋亡指数最低。bcl-2表达与侵袭性高的两个参数：核大小和非整倍体均呈高度显著相关。对核形态、倍性和细胞存活参数进行综合评估，可能有助于在通过细针穿刺活检诊断的早期前列腺癌患者中更好地识别预后不良的患者。
