[核酸核磁共振中的技术问题]

[Technical problems in the nuclear magnetic resonance of nucleic acids].

作者信息

Danchin A

出版信息

Biochimie. 1975;57(4):445-52. doi: 10.1016/s0300-9084(75)80331-2.

Abstract

The application of nuclear magnetic resonance to nucleic acids is discussed here, in terms of the proposed study of their structural, conformational and dynamic aspects. Relaxation techniques, using for example paramagnetic ions as localised probes, provide information about dynamic behaviour ; high resolution spectroscopy about three-dimensional structure, using proton or carbon 13 resonance for the overall study, and phosphorus 31 resonance for the study of phosphate polyribose chain.

摘要

本文讨论了核磁共振在核酸研究中的应用，涉及对核酸结构、构象和动力学方面的研究。弛豫技术，例如使用顺磁性离子作为局部探针，可提供有关动力学行为的信息；高分辨率光谱学用于研究三维结构，利用质子或碳13共振进行整体研究，利用磷31共振研究磷酸多核糖链。