Li X, Wang L, Han X
Department of Cardiology, Gulou Hospital, School of Medicine of Nanjing University, Nanjing 210008 P.R. China.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2001 Aug;18(4):292-5.
To investigate whether angiotensinogen(AGT) M235T, angiotensin II type I receptor(AT(1)R) gene A1166C and angiotensin converting enzyme(ACE) gene I/D polymorphism are implicated in human essential hypertension(HT) in Chinese.
Polymerase chain reaction(PCR) and PCR combined with restriction enzyme digestion were used to detect AGT gene M235T, AT(1)R gene A1166C variations and ACE gene I/D polymorphism in 161 hypertensive patients and 134 normotensive controls.
No statistically significant differences were found in frequencies of the ACE D allele, AGT gene 235T and AT(1)R A1166C between hypertensive patients and normotensive controls, but in hypertensive patients aged <60 years the frequencies of the ACE D allele and AGT gene 235T were significantly higher than those of the normotensive controls (P<0.05). The analysis of combined genotypes of AGT gene and ACE gene showed that the combined genotypes of DD-TT and ID-TT were significantly higher in hypertensive patients than in normotensive controls. Significant relationships between the ACE genotype and serum ACE activity were found in both groups(P<0.05).
The ACE D allele and AGT 235T polymorphism may be involved in the early occurrence of HT. The combined genotypes of DD-TT and ID-TT may be a dangerous genetic factor for HT in Chinese.
研究血管紧张素原（AGT）M235T、血管紧张素Ⅱ1型受体（AT1R）基因A1166C和血管紧张素转换酶（ACE）基因I/D多态性与中国人群原发性高血压（HT）的关系。
采用聚合酶链反应（PCR）及PCR结合限制性内切酶消化法，检测161例高血压患者和134例血压正常对照者的AGT基因M235T、AT1R基因A1166C变异及ACE基因I/D多态性。
高血压患者与血压正常对照者之间，ACE基因D等位基因、AGT基因235T及AT1R基因A1166C的频率差异无统计学意义，但年龄<60岁的高血压患者中，ACE基因D等位基因及AGT基因235T的频率显著高于血压正常对照者（P<0.05）。AGT基因与ACE基因联合基因型分析显示，高血压患者中DD-TT和ID-TT联合基因型显著高于血压正常对照者。两组中均发现ACE基因型与血清ACE活性之间存在显著相关性（P<0.05）。
ACE基因D等位基因及AGT基因235T多态性可能参与高血压的早期发生。DD-TT和ID-TT联合基因型可能是中国人群高血压的一个危险遗传因素。
