人芽囊原虫：程序性细胞死亡过程中细胞内类似半胱天冬酶-3活性的证据。

Blastocystis hominis: evidence for caspase-3-like activity in cells undergoing programmed cell death.

作者信息

Nasirudeen A M, Singh M, Yap E H, Tan K S

机构信息

Department of Microbiology, Faculty of Medicine, National University of Singapore, Singapore.

出版信息

Parasitol Res. 2001 Jul;87(7):559-65. doi: 10.1007/s004360100427.

DOI:10.1007/s004360100427

PMID:11484854

Abstract

We have shown previously that the human intestinal protozoan, Blastocystis hominis, undergoes apoptosis-like programmed cell death (PCD) when exposed to a cytotoxic monoclonal antibody (mAb), 1D5. In the present study, ELISA and immunoblot assays employing chicken anti-CPP32 antibody suggest that caspase-3-like antigens exist in B. hominis. Using colorimetric and flow cytometric assays for caspase-3 activity, we also observed an increase in activity between 1 h and 6 h after exposure to mAb 1D5, with greatest activity at 6 h. These findings suggest that caspase-3-like proteases play an important role in B. hominis undergoing PCD, similar to the phenomenon in higher eukaryotic organisms.

摘要

我们之前已经表明，人类肠道原生动物人芽囊原虫（Blastocystis hominis）在暴露于细胞毒性单克隆抗体（mAb）1D5时会经历凋亡样程序性细胞死亡（PCD）。在本研究中，使用鸡抗CPP32抗体的ELISA和免疫印迹分析表明人芽囊原虫中存在caspase-3样抗原。使用比色法和流式细胞术检测caspase-3活性，我们还观察到在暴露于mAb 1D5后1小时至6小时之间活性增加，在6小时时活性最高。这些发现表明，caspase-3样蛋白酶在人芽囊原虫经历PCD过程中起重要作用，类似于高等真核生物中的现象。