Wang Ke-Xia, Li Chao-Pin, Wang Jian, Cui Yu-Bao
Department of Etiology and Immunology, School of Medicine, Anhui University of Science Technology, Huainan 232001, Anhui Province, China.
World J Gastroenterol. 2002 Oct;8(5):928-32. doi: 10.3748/wjg.v8.i5.928.
To provide scientific evidence for prevention and controlling of blastocystosis, the infection of Blastocystis homonis and to study its clinical significance in Huainan City, Anhui Province, China.
Blastocystis homonis in fresh stools taken from 100 infants, 100 pupils, 100 middle school students and 403 patients with diarrhea was smeared and detected with method of iodine staining and hematoxylin staining. After preliminary direct microscopy, the shape and size of Blastocystis homonis were observed with high power lens. The cellular immune function of the patients with blastocystosis was detected with biotin-streptavidin (BSA).
The positive rates of Blastocystis homonis in fresh stools taken from the infants, pupils, middle school students and the patients with diarrhea, were 1.0 % (1/100), 1.0 % (1/100), 0 % (0/100) and 5.96 % (24/403) respectively. Furthermore, the positive rates of Blastocystis homonis in the stool samples taken from the patients with mild diarrhea, intermediate diarrhea, severe diarrhea and obstinate diarrhea were 6.03 % (14/232), 2.25 % (2/89), 0 % (0/17) and 12.31 % (8/65) respectively. The positive rates of Blastocystis homonis in fresh stools of male and female patients with diarrhea were 7.52 % (17/226) and 3.95 % (7/177) respectively, and those of patients in urban and rural areas were 4.56 % (11/241) and 8.02 % (13/162) respectively. There was no significant difference between them (P>0.05). The positive rates of CD(3)(+), CD(4)(+), CD(8)(+) in serum of Blastocystis homonis-positive and-negative individuals were 0.64+/-0.06, 0.44+/-0.06, 0.28+/-0.04 and 0.60+/-0.05, 0.40+/-0.05 and 0.30+/-0.05 respectively, and the ratio of CD(4)(+)/CD(8)(+) of the two groups were 1.53+/-0.34 and 1.27+/-0.22. There was significant difference between the two groups (P<0.05, P<0.01).
The prevalence of Blastocystis hominis as an enteric pathogen in human seems not to be associated with gender and living environment, and that Blastocystis hominis is more common in stool samples of the patients with diarrhea, especially with chronic diarrhea or obstinate diarrhea. When patients with diarrhea infected by Blastocystis hominis, their cellular immune function decreases, which make it more difficult to be cured.
为安徽省淮南市人体芽囊原虫感染的防治提供科学依据，并探讨其临床意义。
对100名婴儿、100名小学生、100名中学生及403例腹泻患者的新鲜粪便进行涂片，采用碘染色法和苏木精染色法检测人体芽囊原虫。初步直接镜检后，用高倍镜观察人体芽囊原虫的形态和大小。采用生物素-链霉亲和素（BSA）法检测芽囊原虫病患者的细胞免疫功能。
婴儿、小学生、中学生及腹泻患者新鲜粪便中人体芽囊原虫的阳性率分别为1.0%（1/100）、1.0%（1/100）、0%（0/100）和5.96%（24/403）。此外，轻度腹泻、中度腹泻、重度腹泻及顽固性腹泻患者粪便样本中人体芽囊原虫的阳性率分别为6.03%（14/232）、2.25%（2/89）、0%（0/17）和12.31%（8/65）。腹泻男性和女性患者新鲜粪便中人体芽囊原虫的阳性率分别为7.52%（17/226）和3.95%（7/177），城乡患者的阳性率分别为4.56%（11/241）和8.02%（13/162）。两者之间差异无统计学意义（P>0.05）。人体芽囊原虫阳性和阴性个体血清中CD(3)(+)、CD(4)(+)、CD(8)(+)的阳性率分别为0.64±0.06、0.44±0.06、0.28±0.04和0.60±0.05、0.40±0.05和0.30±0.05，两组CD(4)(+)/CD(8)(+)比值分别为1.53±0.34和1.27±0.22。两组之间差异有统计学意义（P<0.05，P<0.01）。
人体芽囊原虫作为肠道病原体在人群中的感染率似乎与性别和生活环境无关，且在腹泻患者尤其是慢性腹泻或顽固性腹泻患者的粪便样本中更为常见。腹泻患者感染人体芽囊原虫后，其细胞免疫功能下降，使得治愈更加困难。
