Erhardt M, Dunoyer P, Guilley H, Richards K, Jonard G, Bouzoubaa S
Institut de Biologie Moléculaire des Plantes, CNRS and Université Louis Pasteur, 12 Rue du Général Zimmer, 67084 Strasbourg Cedex, France.
Virology. 2001 Aug 1;286(2):256-62. doi: 10.1006/viro.2001.0931.
Fluorescent beet necrotic yellow vein virus (BNYVV) particles were produced by replacing part of the readthrough domain of the minor coat protein P75 with the green fluorescent protein (GFP). The recombinant virus was functional in plants and P75-GFP was incorporated at one end of the rod-shaped virions. Laser scanning confocal microscopy and transmission electron microscopy showed that virus-like particles, almost certainly authentic BNYVV virions, localized to the cytoplasmic surface of mitochondria at early times postinfection but relocated at later times to semiordered clusters in the cytoplasm. This is the first report of specific targeting of plant virus particles to the mitochondria in vivo.
通过用绿色荧光蛋白（GFP）替换次要外壳蛋白P75的通读结构域的一部分，产生了荧光甜菜坏死黄脉病毒（BNYVV）颗粒。重组病毒在植物中具有功能，并且P75-GFP被整合到杆状病毒粒子的一端。激光扫描共聚焦显微镜和透射电子显微镜显示，病毒样颗粒几乎可以肯定是真正的BNYVV病毒粒子，在感染后早期定位于线粒体的细胞质表面，但在后期重新定位于细胞质中的半有序簇。这是植物病毒粒子在体内特异性靶向线粒体的首次报道。
