[高效液相色谱法测定食品中的三氯蔗糖]

[Determination of sucralose in foods by HPLC].

作者信息

Kobayashi C, Nakazato M, Yamajima Y, Ohno I, Kawano M, Yasuda K

机构信息

Tokyo Metropolitan Research Laboratory of Public Health: 3-24-1, Hyakunin-cho, Shinjuku-ku, Tokyo 169-0073, Japan.

出版信息

Shokuhin Eiseigaku Zasshi. 2001 Apr;42(2):139-43. doi: 10.3358/shokueishi.42.139.

DOI:10.3358/shokueishi.42.139

PMID:11486381

Abstract

A method for the determination of sucralose in various foods by RI-HPLC and ion chromatography with a pulsed amperometric detector (PAD-IC) was developed. Chopped or homogenized samples were packed into cellulose tubing with 0.01 mol/L hydrochloric acid containing 10% sodium chloride and were dialyzed against 0.01 mol/L hydrochloric acid for 24 hours. The dialyzate was passed through a Bond Elut ENV cartridge, and the cartridge was washed with 0.2 mol/L NaOH and water. Sucralose was eluted from the cartridge with methanol. The extract was taken to dryness in an evaporator and the residue was re-dissolved in water. Sucralose was separated on an Inertsil ODS-3V column with a mobile phase of acetonitrile-water (15:85) and an RI detector. It was also determined on a CarboPak PA1 column with a mobile phase of 100 mmol/L NaOH-75 mmol/L CH3COONa, using a PAD detector. The recoveries of sucralose from various kinds of foods spiked at 50 micrograms/g and 200 micrograms/g ranged from 88-105%. The detection limit in samples was 10 micrograms/g for RI-HPLC and 1 microgram/g for PAD-IC.

摘要

建立了一种采用示差折光检测高效液相色谱法（RI-HPLC）和配备脉冲安培检测器的离子色谱法（PAD-IC）测定各类食品中三氯蔗糖的方法。将切碎或匀浆后的样品装入含有10%氯化钠的0.01 mol/L盐酸的纤维素管中，用0.01 mol/L盐酸透析24小时。透析液通过Bond Elut ENV柱，该柱先用0.2 mol/L氢氧化钠和水冲洗。用甲醇从柱上洗脱三氯蔗糖。提取物在蒸发器中蒸干，残渣用水重新溶解。三氯蔗糖在Inertsil ODS-3V柱上分离，流动相为乙腈-水（15:85），采用示差折光检测器。它也在CarboPak PA-1柱上测定，流动相为100 mmol/L氢氧化钠-75 mmol/L醋酸钠，使用脉冲安培检测器。在添加量为50微克/克和200微克/克的各类食品中，三氯蔗糖的回收率在88%-105%之间。RI-HPLC法对样品的检测限为10微克/克，PAD-IC法为1微克/克。