Acquas E, Di Chiara G
Department of Toxicology, University of Cagliari, Italy.
Neurol Sci. 2001 Feb;22(1):41-2. doi: 10.1007/s100720170037.
In order to determine the role of dopamine (DA) D1 receptors in the control of striatal acetylcholine (ACh) transmission, we studied the effects of SCH 39166 (D1 receptor antagonist), alone or in combination with quinpirole (D2/D3 agonist) or PD 128,907 (D3 agonist) on ACh and DA release. Quinpirole reduced DA and ACh release; PD 128,907 decreased DA but not ACh release. SCH 39166 stimulated DA and decreased ACh release. Pretreatment with quinpirole reduced or prevented (depending on the dose) the stimulation of DA release while potentiating the decrease of ACh release elicited by SCH 39166. Similarly, SCH 39166 administered following PD 128,907 did not stimulate DA release, further decreasing ACh release. These results indicate that quinpirole or PD 128,907 affect the actions of SCH 39166 on DA and ACh release in opposite manner, counteracting the increase of DA release and potentiating the reduction of ACh release. These data support the tenet that endogenous DA exerts a stimulatory input on striatal ACh neurotransmission mediated by D1 receptors.
为了确定多巴胺（DA）D1受体在纹状体乙酰胆碱（ACh）传递控制中的作用，我们研究了SCH 39166（D1受体拮抗剂）单独或与喹吡罗（D2/D3激动剂）或PD 128,907（D3激动剂）联合使用对ACh和DA释放的影响。喹吡罗降低了DA和ACh的释放；PD 128,907降低了DA的释放，但未降低ACh的释放。SCH 39166刺激了DA的释放并降低了ACh的释放。用喹吡罗预处理可降低或阻止（取决于剂量）DA释放的刺激，同时增强SCH 39166引起的ACh释放的降低。同样，在PD 128,907之后给予SCH 39166不会刺激DA释放，反而进一步降低了ACh的释放。这些结果表明，喹吡罗或PD 128,907以相反的方式影响SCH 39166对DA和ACh释放的作用，抵消了DA释放的增加并增强了ACh释放的减少。这些数据支持内源性DA对由D1受体介导的纹状体ACh神经传递施加刺激性输入这一原则。
