Geiss G, Jin G, Guo J, Bumgarner R, Katze M G, Sen G C
Department of Microbiology, University of Washington, Seattle, Washington 98195, USA.
J Biol Chem. 2001 Aug 10;276(32):30178-82. doi: 10.1074/jbc.c100137200.
Double-stranded (ds) RNA, a common component of virus-infected cells, is a potent inducer of the type I interferon and other cellular genes. For identifying the full repertoire of human dsRNA-regulated genes, a cDNA microarray hybridization screening was conducted using mRNA from dsRNA-treated GRE cells. Because these cells lack all type I interferon genes, the possibility of gene induction by autocrine actions of interferon was eliminated. Our screen identified 175 dsRNA-stimulated genes (DSG) and 95 dsRNA-repressed genes. A subset of the DSGs was also induced by different inflammatory cytokines and viruses demonstrating interconnections among disparate signaling pathways. Functionally, the DSGs encode proteins involved in signaling, apoptosis, RNA synthesis, protein synthesis and processing, cell metabolism, transport, and structure. Induction of such a diverse family of genes by dsRNA has major implications in host-virus interactions and in the use of RNA(i) technology for functional ablation of specific genes.
双链（ds）RNA是病毒感染细胞的常见成分，是I型干扰素和其他细胞基因的有效诱导剂。为了鉴定人类dsRNA调控基因的完整清单，使用来自dsRNA处理的GRE细胞的mRNA进行了cDNA微阵列杂交筛选。由于这些细胞缺乏所有I型干扰素基因，排除了干扰素自分泌作用诱导基因的可能性。我们的筛选鉴定出175个dsRNA刺激基因（DSG）和95个dsRNA抑制基因。一部分DSG也由不同的炎性细胞因子和病毒诱导，这表明不同信号通路之间存在相互联系。在功能上，DSG编码参与信号传导、细胞凋亡、RNA合成、蛋白质合成与加工、细胞代谢、运输和结构的蛋白质。dsRNA诱导如此多样的基因家族对宿主-病毒相互作用以及使用RNA干扰（RNAi）技术对特定基因进行功能消除具有重要意义。
