[Cellular compatibility of three natural xenogeneic bone derived biomaterials].

OBJECTIVE

To evaluate the cellular compatibility of three natural xenogeneic bone derived biomaterials.

METHODS

Three types of natural xenogeneic bone derived biomaterials were made with physical and chemical treatment, composite fully deproteinized bone(CFDB), partially deproteinized bone(PDPB) and partially decalcified bone(PDCB). Three types biomaterials were cocultured with human embryonic periosteal osteoblasts. The cell growth, attachment, cell cycle, alkaline phosphatase activity were detected to evaluate the cellular compatibility to biomaterials.

RESULTS

Osteoblasts attached on all three biomaterials and grew well, the effect of three biomaterials on cell proliferation was PDCB > PDPB > CFDB. The cell cycle was not obviously affected by three biomaterials. The effect of three biomaterials on alkaline phosphatase activity of osteoblasts was PDCB > PDPB > CFDB.

CONCLUSION

CFDB,PDPB,PDCB have good cellular compatibility without cytotoxic and tumorigenicity, CFDB is the best. The three biomaterials can be used as scaffold materials of bone tissue engineering.