Despres B, Delseny M, Devic M
Laboratoire Génome et Développement des Plantes, UMR CNRS 5096, Université de Perpignan, 52, Avenue de Villeneuve, 66860 Perpignan-cedex, France.
Plant J. 2001 Jul;27(2):149-59. doi: 10.1046/j.1365-313x.2001.01078.x.
The EMB 506 gene has been characterised as essential for embryo development. To provide insights into the role of EMB 506, which is hidden by the embryo defective phenotype, the ABI3 promoter was fused to the EMB 506 cDNA. The expression of such a transgene should provide sufficient protein during embryogenesis to ensure normal embryo development in homozygous emb 506 seeds. We show that homozygous emb 506 seedlings, partially complemented with the ABI3::EMB 506 transgene, can be obtained. Most of the rescued emb 506 plants are able to flower and to set normal seeds, but show mild to severe depigmentation of rosette leaves and/or inflorescences. This effect on chloroplast development indicated a putative chloroplast localisation of the EMB 506 protein, which was demonstrated by GFP-protein fusion. However, EMB 506 cannot be considered as a chloroplast housekeeping protein only, since EMB 506 is not present in all photosynthetic tissues. This study demonstrates the power of this simple strategy, which could be widely applied to other emb mutants and which may reveal similar or additional roles for EMB genes at vegetative stages of the life cycle.
EMB 506基因已被确定为胚胎发育所必需的基因。为了深入了解被胚胎缺陷表型掩盖的EMB 506的作用，将ABI3启动子与EMB 506 cDNA融合。这种转基因的表达应该在胚胎发生过程中提供足够的蛋白质，以确保纯合emb 506种子的胚胎正常发育。我们表明，可以获得用ABI3::EMB 506转基因部分互补的纯合emb 506幼苗。大多数获救的emb 506植株能够开花并结出正常种子，但莲座叶和/或花序出现轻度至重度色素脱失。这种对叶绿体发育的影响表明EMB 506蛋白可能定位于叶绿体，这通过GFP-蛋白融合得以证实。然而，EMB 506不能仅被视为叶绿体管家蛋白，因为并非所有光合组织中都存在EMB 506。这项研究证明了这种简单策略的有效性，该策略可广泛应用于其他emb突变体，并可能揭示EMB基因在生命周期营养阶段的类似或其他作用。
