Kosai K, Tateyama S, Ikeda T, Uno T, Nishimori T, Takasaki M
Department of Anesthesiology, Miyazaki Medical College, Kiyotake, Miyazaki 889-1692 , Japan.
Brain Res. 2001 Aug 10;910(1-2):12-8. doi: 10.1016/s0006-8993(01)02715-9.
We investigated the role of N-methyl-D-aspartate (NMDA) receptors on non-noxious stimulus-induced pain by examining the effect of MK-801, a non-competitive NMDA receptor antagonist, on Fos-like immunoreactivity (FLI) in the spinal dorsal horn by non-noxious stimulation to rats with chronic constriction injury (CCI) of the sciatic nerve. In CCI rats that did not receive the non-noxious stimulus, FLI was significantly increased in laminae V/VI of the dorsal horn at the 7th and 14th days after surgery relative to sham rats. When CCI rats received non-noxious stimuli, rubbing the plantar of the hind paw, FLI in laminae I/II at the 14th day was significantly increased relative to CCI rats that did not receive the stimulation. In sham rats, the same stimulus significantly decreased FLI in laminae III/IV and V/VI at the 7th and 14th day. When MK-801 was administered intraperitoneally prior to non-noxious stimulation in CCI rats at the 14th day after surgery, the stimulus-induced FLI in laminae I/II in CCI rats was significantly reduced. This study indicates that NMDA receptor is involved in upregulating FLI in response to non-noxious stimulation of CCI rats.
我们通过检测非竞争性N-甲基-D-天冬氨酸（NMDA）受体拮抗剂MK-801对坐骨神经慢性压迫损伤（CCI）大鼠进行非伤害性刺激后脊髓背角中Fos样免疫反应性（FLI）的影响，研究了NMDA受体在非伤害性刺激诱导的疼痛中的作用。在未接受非伤害性刺激的CCI大鼠中，相对于假手术大鼠，术后第7天和第14天背角V/VI层的FLI显著增加。当CCI大鼠接受非伤害性刺激，即摩擦后爪足底时，相对于未接受刺激的CCI大鼠，第14天I/II层的FLI显著增加。在假手术大鼠中，相同刺激在第7天和第14天显著降低了III/IV层和V/VI层的FLI。在术后第14天对CCI大鼠进行非伤害性刺激前腹腔注射MK-801时，CCI大鼠I/II层中刺激诱导的FLI显著降低。本研究表明，NMDA受体参与上调CCI大鼠对非伤害性刺激的FLI。
