Nagao T, Yoshimura S, Saito Y, Nakagomi M, Usumi K, Ono H
Department of Reproductive and Developmental Biology, Hatano Research Institute, Food and Drug Safety Center, Hadano, Kanagawa, Japan.
Reprod Toxicol. 2001 Jul-Aug;15(4):399-411. doi: 10.1016/s0890-6238(01)00141-1.
Sprague-Dawley rats were administered genistein orally at doses of 12.5, 25, 50, or 100 mg/kg on postnatal days 1 through 5 to examine its effects on reproductive function after puberty. In addition, preputial separation and vaginal opening as endpoints of sexual maturation, estrous cycling, sperm count, serum testosterone concentration, and histopathologic changes of reproductive organs of male and female rats were examined. Body weights of male and female rats exposed to genistein at any dose level examined were lower than those of controls. Timing of preputial separation in males and timing of vaginal opening were not affected by genistein treatment. The number of females showing estrous cycle irregularities was increased by genistein treatment. The fertility of female rats exposed neonatally to genistein at 100 mg/kg was disrupted, while neonatal exposure to genistein did not affect male fertility. Neither sperm counts nor serum testosterone concentration were changed by neonatal exposure to genistein. Female rats exposed neonatally to genistein at 100 mg/kg showed histopathologic changes in the ovaries and uterus, while male rats showed no histopathologic alterations in the gonads. The results of this study indicate that early neonatal exposure to genistein caused dysfunction of postpubertal reproductive performance as well as abnormal development of gonads in female but not in male rats.
在出生后第1至5天，给斯普拉格-道利大鼠口服染料木黄酮，剂量分别为12.5、25、50或100毫克/千克，以研究其对青春期后生殖功能的影响。此外，还检查了雄性和雌性大鼠性成熟的终点指标包皮分离和阴道开口、发情周期、精子计数、血清睾酮浓度以及生殖器官的组织病理学变化。在任何检测剂量水平下，接触染料木黄酮的雄性和雌性大鼠的体重均低于对照组。染料木黄酮处理对雄性大鼠的包皮分离时间和雌性大鼠的阴道开口时间没有影响。染料木黄酮处理增加了出现发情周期异常的雌性大鼠数量。新生期接触100毫克/千克染料木黄酮的雌性大鼠的生育能力受到破坏，而新生期接触染料木黄酮对雄性大鼠的生育能力没有影响。新生期接触染料木黄酮对精子计数和血清睾酮浓度均无改变。新生期接触100毫克/千克染料木黄酮的雌性大鼠的卵巢和子宫出现组织病理学变化，而雄性大鼠的性腺未出现组织病理学改变。本研究结果表明，新生期早期接触染料木黄酮会导致雌性大鼠青春期后生殖性能功能障碍以及性腺发育异常，但对雄性大鼠没有影响。
