Hansson H A, Linde A, Nygren H
Cell Tissue Res. 1975 Jun 9;159(2):233-43. doi: 10.1007/BF00219159.
A scanning electron microscopic technique was used to investigate the surface structure of dentinogenically active odontoblasts. Thin pieces of rat incisors were fixed, rapidly frozen, freezedried at minus 70 degrees C and fractured to expose new surfaces prior to examination in the SEM. Differences in the appearance of odontoblastic cell surfaces were seen, with the most extensive ridge formations at the distal part of the sides of the odontoblasts. The predentine area displayed a spongy structure which contrasted to the compact appearance of dentine. Results are discussed in relation to previous studies at the light microscopic and transmission electron miscroscopic levels.
采用扫描电子显微镜技术研究牙本质生成活跃的成牙本质细胞的表面结构。将大鼠切牙薄片固定，快速冷冻，在零下70摄氏度冻干并断裂以暴露新表面，然后在扫描电子显微镜下检查。观察到成牙本质细胞表面外观存在差异，成牙本质细胞侧面远端的嵴形成最为广泛。前期牙本质区域呈现海绵状结构，与牙本质的致密外观形成对比。结合之前在光学显微镜和透射电子显微镜水平的研究对结果进行了讨论。
