Macián M C, Ludwig W, Aznar R, Grimont P A, Schleifer K H, Garay E, Pujalte M J
Int J Syst Evol Microbiol. 2001 Jul;51(Pt 4):1449-1456. doi: 10.1099/00207713-51-4-1449.
Twelve phenotypically similar marine bacteria have been studied by means of ribotyping, DNA-DNA hybridization and cultural and physiological characterization. Phylogenetic analysis has been performed of the 16S and 23S rRNA genes of two representative strains. Phylogenetically, they belong to the Vibrio/Photobacterium branch of the gamma-Proteobacteria and they share all of the properties that define the genus Vibrio. The strains represent a new Vibrio species that is phenotypically similar to Vibrio splendidus. However, resistance to the vibriostatic agent 0129 and production of acid from several carbohydrates allow differentiation between V. splendidus and the proposed new species. The DNA G+C content of the proposed type strain is 44.0 mol %. The name Vibrio lentus sp. nov. is proposed for the new species and strain 40M4T (= CECT 5110T = DSM 13757T) is the type strain.
已通过核糖体分型、DNA-DNA杂交以及培养和生理特征分析,对12种表型相似的海洋细菌进行了研究。对两株代表性菌株的16S和23S rRNA基因进行了系统发育分析。从系统发育角度来看,它们属于γ-变形菌纲的弧菌/发光杆菌分支,并且具有定义弧菌属的所有特性。这些菌株代表了一个新的弧菌物种,其表型与灿烂弧菌相似。然而,对弧菌抑制剂0129的抗性以及从几种碳水化合物产酸的能力使得灿烂弧菌与提议的新物种之间得以区分。提议的模式菌株的DNA G+C含量为44.0摩尔%。提议将新物种命名为迟缓弧菌(Vibrio lentus sp. nov.),菌株40M4T(=CECT 5110T = DSM 13757T)为模式菌株。