Baba S, Hashimoto I, Ishitoya Y, Fukuoka Y
Clin Chim Acta. 1975 Jul 23;62(2):309-14. doi: 10.1016/0009-8981(75)90242-9.
A simple gas chromatographic method for the measurement of low molecular weight urinary monoamines has been developed without use of a prior isolation procedure. The primary and secondary monamines in urine were directly converted into their 2,4-dinitrophenyl derivatives with 2,4-dinitrobenzensulfonate in aqueous alkaline solution, which after extraction with benzene and concentration, were submitted to gas chromatography. The application of this method to the determination of methylamine and dimethylamine in urine from normal subjects and hepatic patients is described.
已开发出一种简单的气相色谱法，用于测定低分子量尿单胺，无需事先进行分离程序。尿液中的伯胺和仲胺在碱性水溶液中直接与2,4-二硝基苯磺酸盐反应转化为其2,4-二硝基苯基衍生物，经苯萃取和浓缩后，进行气相色谱分析。描述了该方法在正常人和肝病患者尿液中甲胺和二甲胺测定中的应用。
