Timm S, Otto C, Hamelmann W, Gassel A M, Timmermann W, Ulrichs K, Thiede A
Department of Surgery, University of Würzburg, Würzburg, Germany.
Microsurgery. 2001;21(5):221-2. doi: 10.1002/micr.1042.
MHC antigen expression in parathyroid tissue and its influence on graft survival after allogeneic transplantation were investigated using a heterotopic rat transplantation model. MHC class I and II expression in parathyroid tissue of Lewis (LEW), Dark Agouti (DA), and Wistar-Furth (WF) rats was analysed semi-quantitatively by using immunohistochemistry. MHC class I expression was strong in DA, moderate in WF, and weak in LEW rats parenchyma, whereas MHC class II expression was negative. In the interstitium of all investigated tissue specimens, the proportion of MHC class II-expressing cells was low. Additionally, four groups were transplanted: 1) LEW to LEW, 2) DA to DA, 3) LEW to DA, and 4) WF to LEW. After syngeneic transplantation, graft survival could be documented over the whole observation period. A median graft survival of 20 (+/-2) days was observed after transplantation from LEW to DA, whereas grafts in the group WF to LEW were rejected after 13 (+/-1) days. The number of intra-graft leucocytes expressing MHC class II molecules was the same in all groups, whereas increased levels of MHC class I in parathyroid tissue before transplantation resulted in a more rapid rejection. These results indicate that immunogenicity of rat parathyroid tissue might be determined by the amount of MHC class I expressed in donor parenchymal cells. Further experiments are necessary to validate this observation.
利用异位大鼠移植模型，研究了甲状旁腺组织中的MHC抗原表达及其对同种异体移植后移植物存活的影响。采用免疫组织化学方法对Lewis（LEW）、Dark Agouti（DA）和Wistar-Furth（WF）大鼠甲状旁腺组织中的MHC I类和II类表达进行了半定量分析。MHC I类在DA大鼠实质中表达强，在WF大鼠中表达中等，在LEW大鼠中表达弱，而MHC II类表达为阴性。在所有研究的组织标本间质中，表达MHC II类的细胞比例较低。此外，进行了四组移植：1）LEW到LEW，2）DA到DA，3）LEW到DA，4）WF到LEW。同基因移植后，在整个观察期内均可记录移植物存活情况。LEW到DA移植后观察到移植物中位存活时间为20（±2）天，而WF到LEW组的移植物在13（±1）天后被排斥。所有组中表达MHC II类分子的移植物内白细胞数量相同，而移植前甲状旁腺组织中MHC I类水平升高导致排斥反应更快。这些结果表明，大鼠甲状旁腺组织的免疫原性可能由供体实质细胞中表达的MHC I类量决定。需要进一步的实验来验证这一观察结果。
