Vanore G, Giraldez L, Rodríguez de Lores Arnaiz G, Girardi E
Instituto de Biologia Celular y Neurociencias Prof. E. De Robertis, Facultad de Medicina, Universidad de Buenos Aires, Argentina.
Neurochem Res. 2001 Mar;26(3):225-30. doi: 10.1023/a:1010912516299.
Specific ligand binding to rat hippocampal adenosine A1 receptor after administration of the convulsant drug 3-mercaptopropionic acid (MP) was studied by means of a quantitative autoradiographic method. 2-Chloro-N6-[cyclopentyl-2,3,4,5-3H adenosine] ([3H]CCPA), a potent and selective A1 receptor ligand, was selected for binding studies. MP administration (150 mg/kg, i.p.), at seizure, caused significant increases in the following CA1 layers: pyramidal (45%), radiatum (18%) and lacunosum molecular (35%); in CA2 area, a significant decrease in stratum oriens (36%) and an increase in stratum radiatum (14%) and lacunosum molecular (33%) layers was observed. In CA3 area a rise in pyramidal (40%) and radiatum layers (26%), as well as in hillus (97%) was found. At postseizure, changes were restricted to CA1, CA2 and CA3 pyramidal layers and to CA1 lacunosum molecular layer, with increases ranging from 22 to 50%. These results show that [3H]CCPA binding is modified diversely in intrahippocampal layers and areas, thus indicating their dissimilar role in seizure activity.
采用定量放射自显影法研究了惊厥药3-巯基丙酸（MP）给药后大鼠海马腺苷A1受体的特异性配体结合情况。选择强效且选择性的A1受体配体2-氯-N6-[环戊基-2,3,4,5-3H腺苷]（[3H]CCPA）进行结合研究。在癫痫发作时腹腔注射MP（150mg/kg）后，CA1层的以下区域出现显著增加：锥体层（45%）、辐射层（18%）和分子层（35%）；在CA2区域，观察到原层显著减少（36%），辐射层增加（14%），分子层增加（33%）。在CA3区域，锥体层（40%）、辐射层（26%）以及海马齿状回（97%）出现增加。癫痫发作后，变化仅限于CA1、CA2和CA3锥体层以及CA1分子层，增加幅度在22%至50%之间。这些结果表明，[3H]CCPA结合在海马内各层和区域发生不同程度的改变，从而表明它们在癫痫活动中发挥不同作用。
