（Nphe1）孤啡肽（1-13）-NH₂对鞘内注射孤啡肽诱导的大鼠抗伤害感受的阻断作用

Blockade effects of (Nphe1)Nociceptin(1-13)-NH(2) on anti-nociception induced by intrathecal administration of nociceptin in rats.

作者信息

Lu J T, Huang Y H, Palmer P P, Xie G X, Gabriel A, Grond S, Yu L C

机构信息

Department of Physiology, College of Life Sciences, Peking University, 100871, Beijing, China.

出版信息

Regul Pept. 2001 Sep 15;101(1-3):81-5. doi: 10.1016/s0167-0115(01)00263-4.

DOI:10.1016/s0167-0115(01)00263-4

PMID:11495682

Abstract

The present study investigated the roles of the opioid-receptor-like (ORL1) receptor and its endogenous ligand nociceptin on nociception in the spinal cord of rats. Intrathecal administration of 10 nmol of nociceptin produced significant increases in hindpaw withdrawal latencies (HWLs) to thermal and mechanical stimulation. There were no significant changes of average maximum angles in inclined plane tests after intrathecal injection of 10 nmol of nociceptin in rats. The intrathecal nociceptin-induced increases in HWL were antagonized by intrathecal administration of (Nphe1)Nociceptin(1-13)-NH(2), a selective antagonist of ORL1 receptor, in a dose-dependent manner. The results demonstrated that ORL1 receptor is involved in the nociceptin-induced anti-nociceptive effect in the spinal cord of rats.

摘要

本研究调查了阿片样物质受体样（ORL1）受体及其内源性配体孤啡肽在大鼠脊髓痛觉感受中的作用。鞘内注射10 nmol孤啡肽可使后爪对热刺激和机械刺激的缩爪潜伏期（HWL）显著增加。大鼠鞘内注射10 nmol孤啡肽后，倾斜平面试验中的平均最大角度无显著变化。鞘内注射ORL1受体选择性拮抗剂（Nphe1）孤啡肽（1-13）-NH₂可剂量依赖性地拮抗鞘内注射孤啡肽引起的HWL增加。结果表明，ORL1受体参与了孤啡肽在大鼠脊髓中诱导的抗痛觉效应。