Butter N L, Hattersley A T, Clark P M
Regional Endocrine Laboratory, Department of Clinical Biochemistry, University Hospital Birmingham NHS Trust, Selly Oak Hospital, Raddlebarn Road, Birmingham B29 6JD, UK.
Clin Chim Acta. 2001 Aug 20;310(2):141-50. doi: 10.1016/s0009-8981(01)00545-9.
Bloodspot assays for hormones have advantages of ease of collection/transportation, minimal invasiveness and small blood volume. Such an approach would be of use in epidemiological studies of insulin resistance in children and neonates.
A two-site chemiluminescent immunoassay for the quantitation of insulin in bloodspots dried on filter paper, based on modifications of a commercially available kit, was used. Analysis was carried out on two 3-mm diameter discs punched out from whole blood standards and test samples.
The detection limit of the assay was 5.9 pmol/l of whole blood, with mean intra-assay CV and mean inter-assay CV being <15% above 24 pmol/l. The mean recovery of added insulin was 83%. Comparison of paired whole bloodspots and serum samples collected simultaneously gave a correlation of 0.89. The bloodspot insulin concentrations were stable in excess of 2 months when stored at -20 degrees C and were not susceptible to degradation after four freeze-thaw cycles.
This simple and convenient method is suitable for the measurement of insulin in small volumes of blood collected on filter paper cards and can be applied to epidemiological studies of insulin resistance.
激素血斑检测具有采集/运输方便、微创性和血量少等优点。这种方法将用于儿童和新生儿胰岛素抵抗的流行病学研究。
基于对市售试剂盒的改进，采用一种用于定量滤纸干血斑中胰岛素的双位点化学发光免疫分析法。对从全血标准品和测试样品中冲出的两个直径3毫米的圆盘进行分析。
该检测方法的检测限为全血5.9 pmol/l，在24 pmol/l以上时，批内变异系数均值和批间变异系数均值均<15%。添加胰岛素的平均回收率为83%。同时采集的配对全血斑和血清样品的比较显示相关性为0.89。血斑胰岛素浓度在-20℃储存时超过2个月稳定，经过4次冻融循环后不易降解。
这种简单方便的方法适用于测量滤纸片上采集的少量血液中的胰岛素，可应用于胰岛素抵抗的流行病学研究。
