Brand R C, Klootwijk J, Sibum C P, Planta R J
Nucleic Acids Res. 1979 Sep 11;7(1):121-34. doi: 10.1093/nar/7.1.121.
The pseudouridylation of ribosomal RNA of Saccharomyces carlsbergensis was investigated with respect to its timing during the maturation of rRNA and its sequence specificity. Analysis of 37-S RNA, the common precursor to 17-S, 5.8-S and 26-S rRNA and most probably the primary ribosomal transcript, shows that this RNA molecule contains already most if not all of the 36-37 pseudouridine residues found in the mature rRNAs. Thus pseudouridylation is, like 2'-0-ribosemethylation, an early event in the maturation of rRNA, taking place immediately after, or even during, transcription. The data presented show that the non-conserved sequences of 37-S precursor rRNA contain very few pseudouridine residues if any. The pseudouridine residues within the rRNA sequences are apparently clustered to a certain degree as can inferred from the occurrence of a single oligonucleotide containing 3 pseudouridines, which was obtained by digestion of 26-S rRNA with ribonuclease T1.
就核糖体RNA（rRNA）假尿苷酸化在rRNA成熟过程中的时间安排及其序列特异性，对卡尔斯伯酵母核糖体RNA的假尿苷酸化进行了研究。对37 - S RNA（17 - S、5.8 - S和26 - S rRNA的共同前体，很可能也是核糖体的初级转录本）的分析表明，该RNA分子已经含有成熟rRNA中发现的36 - 37个假尿苷残基中的大部分（如果不是全部的话）。因此，假尿苷酸化与2'-O-核糖甲基化一样，是rRNA成熟过程中的早期事件，在转录后立即发生，甚至在转录过程中发生。所呈现的数据表明，37 - S前体rRNA的非保守序列中即使有也很少有假尿苷残基。从用核糖核酸酶T1消化26 - S rRNA得到的一个含有3个假尿苷的单一寡核苷酸的出现可以推断，rRNA序列中的假尿苷残基明显在一定程度上聚集在一起。
