Zhu D L, Gogusev J, Marche P
Shanghai Institute of Hypertension, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025.
Sheng Li Xue Bao. 1999 Apr;51(2):181-6.
The resulats of this study are as follows. (1) As measured by a bioassay, a macrophage colony-stimulating activity was detected in the serum-free conditioned medium of rat aortic vascular smooth muscle cells (VSMCs), which could be subdued by the addition of specific anti macrophage colony-stimulating factor (MCSF) antibody. (2) The presence of MCSF receptor was confirmed by immunocytochemistry using a specific anti c-Fms antibody. (3) The presence of mRNAs for MCSF and c-fms (which encoded MCSF receptor) was determined by Northern blot analysis. Their expressions were detectable in quiescent VSMCs and markedly increased after addition of serum. These data demonstrated for the first time the production of MCSF and the presence of MCSF receptor in cultured rat VSMCs. It is suggested that MCSF might modulate VSMCs functions via both autocrine and paracrine mechanisms. Rat VSMCs appear to be a suitable cell model for studying the cell proliferation effect of MCSF.
本研究结果如下。（1）通过生物测定法检测发现，大鼠主动脉血管平滑肌细胞（VSMC）的无血清条件培养基中存在巨噬细胞集落刺激活性，添加特异性抗巨噬细胞集落刺激因子（MCSF）抗体可使其受到抑制。（2）使用特异性抗c-Fms抗体通过免疫细胞化学法证实了MCSF受体的存在。（3）通过Northern印迹分析确定了MCSF和c-fms（编码MCSF受体）的mRNA的存在。在静止的VSMC中可检测到它们的表达，添加血清后表达明显增加。这些数据首次证明了培养的大鼠VSMC中MCSF的产生及MCSF受体的存在。提示MCSF可能通过自分泌和旁分泌机制调节VSMC的功能。大鼠VSMC似乎是研究MCSF细胞增殖作用的合适细胞模型。
