Lees B J, Brighton W D
Dev Biol Stand. 1975;29:227-34.
Methods of measuring histamine from large numbers of cells involve inaccuracies due to the difficulty of isolating a pure cell population without damage to the cells. A new method of measuring histamine within single cells by a microspectrophotofluorimetric method has been developed. Calibration of the microspectrophotofluorimeter used o-phtalaldehyde-conjugated-histamine absorbed to Sephadex particles. The concentration of histamine within single rat mast cells was measured and compared with the theoretical values obtained from measuring histamine released from large cell samples.
从大量细胞中测量组胺的方法存在不准确之处，因为要分离出纯净的细胞群体且不损伤细胞很困难。一种通过显微分光光度荧光法在单细胞内测量组胺的新方法已经开发出来。所使用的显微分光光度计用吸附在葡聚糖凝胶颗粒上的邻苯二甲醛结合组胺进行校准。测量了单个大鼠肥大细胞内组胺的浓度，并与从大细胞样本中释放的组胺测量得到的理论值进行了比较。
