Gan S H, Ismail R
Pharmacology Department, School of Medical Sciences, University Sains Malaysia, Kerian Kelantan.
J Chromatogr B Biomed Sci Appl. 2001 Aug 15;759(2):325-35. doi: 10.1016/s0378-4347(01)00237-7.
An HPLC system using solid-phase extraction and HPLC with UV detection has been validated in order to determine tramadol and o-desmethyltramadol (M1) concentrations in human plasma. The method developed was selective and linear for concentrations ranging from 50 to 3,500 ng/ml (tramadol) and 50 to 500 ng/ml (M1) with mean recoveries of 94.36 +/- 12.53% and 93.52 +/- 7.88%, respectively. Limit of quantitation (LOQ) was 50 ng/ml. For tramadol, the intra-day accuracy ranged from 95.48 to 114.64% and the inter-day accuracy, 97.21 to 103.24%. Good precision (0.51 and 18.32% for intra- and inter-day, respectively) was obtained at LOQ. The system has been applied to determine tramadol concentrations in human plasma samples for a pharmacokinetic study.
已验证一种采用固相萃取和带紫外检测的高效液相色谱法（HPLC）的系统，用于测定人血浆中曲马多和O-去甲基曲马多（M1）的浓度。所开发的方法对于50至3500 ng/ml（曲马多）和50至500 ng/ml（M1）的浓度具有选择性和线性，平均回收率分别为94.36±12.53%和93.52±7.88%。定量限（LOQ）为50 ng/ml。对于曲马多，日内准确度范围为95.48%至114.64%，日间准确度范围为97.21%至103.24%。在定量限处获得了良好的精密度（日内和日间分别为0.51%和18.32%）。该系统已应用于药代动力学研究中测定人血浆样品中的曲马多浓度。
