无支架猪生物瓣膜的抗矿化处理：一项实验研究。

Antimineralization treatments in stentless porcine bioprostheses: an experimental study.

作者信息

Flameng W, Ozaki S, Meuris B, Herijgers P, Yperman J, Van Lommel A, Verbeken E

机构信息

Department of Cardiac Surgery, Katholieke Universiteit Leuven, Belgium.

出版信息

J Heart Valve Dis. 2001 Jul;10(4):489-94.

PMID:11499596

Abstract

BACKGROUND AND AIM OF THE STUDY

Photo-oxidation treatment of porcine stentless bioprostheses (Photofix) was compared with glutaraldehyde fixation, with either AOA (Freestyle valve) or Tween-80 (Edwards Prima Plus valve).

METHODS

Six valves of each type were implanted in juvenile sheep, in the pulmonary position. Valves were explanted after three or six months and examined macroscopically, by X-radiography, and by light and transmission electron microscopy. Calcium content was determined by atomic absorption spectrometry.

RESULTS

The cusps of all valves were free of calcification, and had normal histology and function. Calcium contents (median +/- IQR) were 0.63+/-0.45, 0.73+/-1.46 and 0.46+/-1.42 microg/mg for the Photofix, Freestyle and Prima Plus valves, respectively (p = NS). Calcium contents of the aortic wall portions were 0.71+/-1.27 (Photofix), 10.78+/-77.22 (Freestyle) and 28.70+/-66.53 (Prima Plus) (p <0.05 for Photofix versus Freestyle or Prima Plus).

CONCLUSION

Photo-oxidation of a porcine stentless valve prevents calcification not only in the cusps, but also in the aortic wall portion.

摘要

研究背景与目的

将猪无支架生物瓣膜的光氧化处理（Photofix）与戊二醛固定处理进行比较，戊二醛固定处理采用AOA（Freestyle瓣膜）或吐温80（Edwards Prima Plus瓣膜）。

方法

每种类型的六个瓣膜植入幼年绵羊的肺动脉位置。三个月或六个月后取出瓣膜，进行宏观检查、X射线摄影、光镜和透射电镜检查。通过原子吸收光谱法测定钙含量。

结果

所有瓣膜的瓣叶均无钙化，组织学和功能正常。Photofix瓣膜、Freestyle瓣膜和Prima Plus瓣膜的钙含量（中位数±四分位间距）分别为0.63±0.45、0.73±1.46和0.46±1.42微克/毫克（p =无显著性差异）。主动脉壁部分的钙含量分别为0.71±1.27（Photofix）、10.78±77.22（Freestyle）和28.70±66.53（Prima Plus）（Photofix与Freestyle或Prima Plus相比，p<0.05）。