BIACORE J：用于常规生物分子相互作用分析的新平台。

BIACORE J: a new platform for routine biomolecular interaction analysis.

作者信息

Rich R L, Myszka D G

机构信息

Center for Biomolecular Interaction Analysis, School of Medicine, University of Utah, Salt Lake City, UT 84132, USA.

出版信息

J Mol Recognit. 2001 Jul-Aug;14(4):223-8. doi: 10.1002/jmr.535.

DOI:10.1002/jmr.535

PMID:11500968

Abstract

SPR biosensor technology continues to evolve. The recently released platform from Biacore AB (Uppsala, Sweden), BIACORE J, is designed for the routine analysis of biomolecular interactions. Using an antibody-protein A and a ligand-receptor system, we demonstrate the utility of BIACORE J in determining active concentration and binding affinities. The results from these studies illustrate the high sensitivity of the instrument and its ability to generate reproducible binding responses. The BIACORE J is easy to operate and useful in diverse applications, making SPR technology widely accessible as a research tool.

摘要

表面等离子体共振（SPR）生物传感器技术不断发展。最近由瑞典乌普萨拉的Biacore AB公司发布的BIACORE J平台，专为生物分子相互作用的常规分析而设计。通过使用抗体-蛋白A和配体-受体系统，我们证明了BIACORE J在确定活性浓度和结合亲和力方面的实用性。这些研究结果表明了该仪器的高灵敏度及其产生可重复结合响应的能力。BIACORE J易于操作，适用于多种应用，使得SPR技术作为一种研究工具广泛可用。