[Human preadipocyte culture and the establishment of hyperplasia and hypertrophy model].

OBJECTIVE

To better understand hyperplasia and hypertrophy properties of human adipose tissue.

METHODS

Using an "adhesion-ceiling" culture strategy, fibroblast-like cells from adult pure adipose granules were successfully cultured.

RESULTS

The cells were highly homogeneous, highly proliferative and with a high differentiation rate. Their dynamic morphological changes, growth curve, glycerophosphate dehydrogenase pattern, oil red O staining, and reaction to insulin and dexamethasone all verified their preadipocyte identity. Under controlled conditions, the preadipocytes replayed their in vivo hyperplasia and hypertrophy process.

CONCLUSION

In mature human adipose tissue there exist functionally active fractions that can be modified. This study laid the basis for further probing into obesity and fat autotransplantation.