[Experimental study of cultured autologous keratinocyte sheet(Auto-CKS) and allodermis composite skin grafted onto open wound of rats].

OBJECTIVE

Investigate the "take" and histological changes of grafted auto-CKS and allo-dermis composite skin.

METHODS

Thirty Sprague-Dawley rats were divided into group auto-CKS and group allo-dermis composite skin. All wounds were dressed after grafting. Wound healing and "take" of grafts were determined for 90 days after grafting. Biopsies of wound tissue including grafts were collected at regular intervals for histological analysis to check the reconstruction of epidermis-dermis junction (EDJ) and fate of allo-dermis of the composite skin.

RESULTS

In group auto-CKS, the "take" of grafts was pretty good, but the quality and histological structure of CKS was not good enough, the proliferated collagen fibers were disorderly arranged, and the CKS was too thin to withstand abrasion and tended to worn out and contracted severely. On the other hand, the "take" as well as the quality, histological structure, abrasion resistance and contraction of composite skin in the allo-dermis composite skin group were much better than that of group auto-CKS. Up to 90 days after grafting, the allo-dermis still existed without obvious signs of immuno-rejection.

CONCLUSION

The CKS was not suitable for the wound of full thickness skin defect, while the composite skin composed of auto-CKS and treated allo-dermis unfolded the potential encouraging prospects.