[金属硫蛋白（MT）对大鼠烧伤皮肤的影响]
[Effects of metallothionein (MT) on burned skin of rats].
作者信息
Yu D, Qin F, Sun Y
机构信息
Department of Burns, Beijing Jishuitan Hospital, Beijing 100035.
出版信息
Zhonghua Zheng Xing Shao Shang Wai Ke Za Zhi. 1999 Mar;15(2):92-4.
OBJECTIVE
To elucidate the relationship between skin viability and lipid peroxidation after deep partial thickness burn injury.
METHODS
Deep partial thickness burn was produced. Metallothionin (MT) in different concentrations (0.5 x 10(-6) mol/L, 1 x 10(-5) mol/L) was topically applied. Oxygen consumption, succinate dehydrogenase(SDH), Schiff's base contents and healing time of wound were determined in 24 Wistar rats. Specimens were harvested at 8, 24, and 48-hour postburn.
RESULTS
Oxygen consumption and SDH value decreased and Schiff's base value increased after burn injury. With the application of MT, oxygen consumption and SDH value of burned skin were significantly higher compared with control group, and Schiff's base value was significantly lower (P < 0.05 or P < 0.01). The mean healing time of burned wound was two days less in two MT protection groups.
CONCLUSION
MT may be beneficial in improving postburn skin viability by attenuating lipid peroxidation of burned tissue.
目的
阐明深Ⅱ度烧伤后皮肤活力与脂质过氧化之间的关系。
方法
制作深Ⅱ度烧伤模型。对24只Wistar大鼠局部应用不同浓度（0.5×10⁻⁶mol/L、1×10⁻⁵mol/L）的金属硫蛋白（MT）。测定伤后24小时的耗氧量、琥珀酸脱氢酶（SDH）、席夫碱含量及伤口愈合时间。于烧伤后8小时、24小时和48小时采集标本。
结果
烧伤后耗氧量及SDH值降低，席夫碱值升高。应用MT后，烧伤皮肤的耗氧量及SDH值明显高于对照组，席夫碱值明显低于对照组（P<0.05或P<0.01）。两个MT保护组的烧伤创面平均愈合时间缩短两天。
结论
MT可能通过减轻烧伤组织的脂质过氧化而有助于改善烧伤后皮肤活力。
Zotero 插件