丁酸弧菌属的多种内切木聚糖酶

Multiple endoxylanases of Butyrivibrio sp.

作者信息

Zorec M, Cepeljnik T, Nekrep F V, Logar R M

机构信息

Zootechnical Department, Biotechnical Faculty, University of Ljubljana, 1230 Domzale, Slovenia.

出版信息

Folia Microbiol (Praha). 2001;46(1):94-6. doi: 10.1007/BF02825897.

DOI:10.1007/BF02825897

PMID:11501489

Abstract

Butyrivibrio sp. Mz 5 with a high xylanolytic activity was isolated. Four major xylanases were detected in the cell-associated fraction using the zymogram technique. The xylanolytic activity was inducible with the oat spelts xylan; two endoxylanases (51 and 145 kDa) were formed constitutively. The bulk of the xylanolytic activity was cell-bound and growth-phase dependent; the maximum activity in the cell-associated fraction was achieved after 16 h of incubation. The highest xylanolytic activity was determined in a medium with 0.5% oat spelts xylan. Under optimum conditions (the highest xylanolytic activity produced), the two cell-bound xylanases (51 and 58 kDa) were isolated by anion exchange chromatography on CIM DEAE 8 tubes attached to a MPLC system, and gel filtration.

摘要

分离出具有高木聚糖酶活性的丁酸弧菌Mz 5。使用酶谱技术在细胞相关部分检测到四种主要木聚糖酶。木聚糖酶活性可被燕麦麸木聚糖诱导；两种内切木聚糖酶（51 kDa和145 kDa）组成型形成。大部分木聚糖酶活性与细胞结合且依赖生长阶段；细胞相关部分在培养16小时后达到最大活性。在含有0.5%燕麦麸木聚糖的培养基中测定到最高木聚糖酶活性。在最佳条件下（产生最高木聚糖酶活性），通过连接到MPLC系统的CIM DEAE 8管上的阴离子交换色谱和凝胶过滤分离出两种细胞结合木聚糖酶（51 kDa和58 kDa）。