Skibiński R, Misztal G
Department of Medicinal Chemistry, Medical Academy, Lublin, Poland.
Acta Pol Pharm. 2001 Mar-Apr;58(2):97-100.
A simple, accurate high performance liquid chromatography procedure is presented in order to determine moclobemide in Aurorix-tablets, paroxetine in Seroxat-tablets and fluvoxamine in Fevarin tablets. These drugs were chromatographed on a Nova-Pak C18, (dp = 4 microm), 150x3.9 mm i.d. column using methanol/phosphate butter adjusted to pH 2.65 with phosphoric acid (7:3, v/v) as the mobile phase to determining of the moclobemide and methanol/tetrahydrofuran/phosphate buffer at pH 2.65 (53:5:4, v/v) for determining of paroxetine and fluvoxamine. The detection was carried at 235 nm. The method was tested for linearity over the range 5-50 microg/ml. The relative standard deviation for moclobemide is 1.16, but for paroxetine and fluvoxamine is less than 1% (0.25 and 0.46 respectively).
为测定奥洛他定片中的吗氯贝胺、帕罗西汀片中的帕罗西汀和氟伏沙明片中的氟伏沙明，介绍了一种简单、准确的高效液相色谱法。这些药物在Nova-Pak C18（粒径4微米）、内径150×3.9毫米的色谱柱上进行色谱分析，以用磷酸调节至pH 2.65的甲醇/磷酸盐缓冲液（7:3，v/v）作为流动相来测定吗氯贝胺，用pH 2.65的甲醇/四氢呋喃/磷酸盐缓冲液（53:5:4，v/v）来测定帕罗西汀和氟伏沙明。检测波长为235纳米。该方法在5-50微克/毫升范围内进行线性测试。吗氯贝胺的相对标准偏差为1.16，而帕罗西汀和氟伏沙明的相对标准偏差小于1%（分别为0.25和0.46）。
